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Assay Detail

CHEMBL3583408

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to biotinylated BRD2 BD2 (349 to 460 amino acid residues) (unknown origin) expressed in Rosetta2 DE3 cells by bio-layer interferometry method
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bromodomain-containing protein 2 (CHEMBL1293289)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-Based Design of γ-Carboline Analogues as Potent and Specific BET Bromodomain Inhibitors.
Ran X, Zhao Y, Liu L, Bai L, Yang CY, Zhou B, Meagher JL, Chinnaswamy K, Stuckey JA, Wang S.
J Med Chem (2015) 58 : 4927 -4939
PubMed 26080064 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 4 7.56 8.21

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1957266 1 8.21
CHEMBL3581661 1 7.53
CHEMBL1232461 MOLIBRESIB 2.0 1 7.34
CHEMBL2017291 1 7.15

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1957266 Kd = 6.2 nM 8.21
CHEMBL3581661 Kd = 29.6 nM 7.53
CHEMBL1232461 MOLIBRESIB Kd = 45.4 nM 7.34
CHEMBL2017291 Kd = 70.3 nM 7.15