Assay Detail
Binding
CHEMBL3583772
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Inhibition of human DNA topoisomerase-1 using supercoiled pBAD-GFPuv plasmid DNA as substrate assessed as prevention of supercoiled DNA template from relaxation after 30 mins by agarose gel electrophoresis
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Selective Inhibition of Bacterial Topoisomerase I by alkynyl-bisbenzimidazoles.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 6 | 4.62 | 4.64 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL301735 | HOECHST 33258 | — | 1 | 4.64 |
| CHEMBL3581009 | — | — | 1 | 4.59 |
| CHEMBL3581012 | — | — | 1 | - |
| CHEMBL3581011 | — | — | 1 | - |
| CHEMBL3581010 | — | — | 1 | - |
| CHEMBL343002 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL301735 | HOECHST 33258 | IC50 | = | 22860.0 | nM | 4.64 |
| CHEMBL3581009 | — | IC50 | = | 25410.0 | nM | 4.59 |
| CHEMBL3581012 | — | IC50 | > | 50000.0 | nM | - |
| CHEMBL3581011 | — | IC50 | > | 50000.0 | nM | - |
| CHEMBL3581010 | — | IC50 | > | 50000.0 | nM | - |
| CHEMBL343002 | — | IC50 | > | 50000.0 | nM | - |