Assay Detail
Binding
CHEMBL3583773
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human DNA topoisomerase-2 using supercoiled pBAD-GFPuv plasmid DNA as substrate assessed as prevention of supercoiled DNA template from relaxation after 30 mins by agarose gel electrophoresis
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Selective Inhibition of Bacterial Topoisomerase I by alkynyl-bisbenzimidazoles.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 2 | 4.42 | 4.54 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL301735 | HOECHST 33258 | — | 1 | 4.54 |
| CHEMBL3581009 | — | — | 1 | 4.29 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL301735 | HOECHST 33258 | IC50 | = | 28920.0 | nM | 4.54 |
| CHEMBL3581009 | — | IC50 | = | 51600.0 | nM | 4.29 |