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Assay Detail

CHEMBL3583910

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
In vitro inhibition of full length human ACAT1 transfected in Sf-21 cells using [1-14C]oleoyl-CoA assessed as incorporation of radioactivity into lipid by TLC assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf21
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sterol O-acyltransferase 1 (CHEMBL2782)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a Novel Series of N-Phenylindoline-5-sulfonamide Derivatives as Potent, Selective, and Orally Bioavailable Acyl CoA:Monoacylglycerol Acyltransferase-2 Inhibitors.
Sato K, Takahagi H, Yoshikawa T, Morimoto S, Takai T, Hidaka K, Kamaura M, Kubo O, Adachi R, Ishii T, Maki T, Mochida T, Takekawa S, Nakakariya M, Amano N, Kitazaki T.
J Med Chem (2015) 58 : 3892 -3909
PubMed 25897973 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 4.46 4.46

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3581716 1 4.46

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3581716 IC50 = 35000.0 nM 4.46