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Assay Detail

CHEMBL3584412

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Anti-inflammatory activity in mouse RAW264.7 cells assessed as LPS-stimulated inhibition of nitric oxide production incubated simultaneously with LPS for 24 hrs by Griess reagent based assay
15
Total Activities
15
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type RAW264.7
Confidence 1 — Organism
Curated By Autocuration

Target

RAW264.7 (CHEMBL612557)
Type CELL-LINE
Organism Mus musculus

Publication

Ervatamines A-I, Anti-inflammatory Monoterpenoid Indole Alkaloids with Diverse Skeletons from Ervatamia hainanensis.
Zhang DB, Yu DG, Sun M, Zhu XX, Yao XJ, Zhou SY, Chen JJ, Gao K.
J Nat Prod (2015) 78 : 1253 -1261
PubMed 26024020 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Inhibition 12 - -
IC50 3 4.45 4.59

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL364613 CORONARIDINE 1 4.59
CHEMBL3580947 1 4.38
CHEMBL6 INDOMETHACIN 4.0 1 4.37
CHEMBL3580944 1 -
CHEMBL3580943 1 -
CHEMBL3580942 1 -
CHEMBL4524091 1 -
CHEMBL3580940 1 -
CHEMBL3580939 1 -
CHEMBL3580938 1 -
CHEMBL3580937 1 -
CHEMBL3580936 1 -
CHEMBL461609 HEYNEANINE 1 -
CHEMBL3580945 1 -
CHEMBL3580946 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL364613 CORONARIDINE IC50 = 25500.0 nM 4.59
CHEMBL3580947 IC50 = 41500.0 nM 4.38
CHEMBL6 INDOMETHACIN IC50 = 42600.0 nM 4.37
CHEMBL3580944 Inhibition - % -
CHEMBL3580943 Inhibition - % -
CHEMBL3580942 Inhibition - % -
CHEMBL4524091 Inhibition - % -
CHEMBL3580940 Inhibition - % -
CHEMBL3580939 Inhibition - % -
CHEMBL3580938 Inhibition - % -
CHEMBL3580937 Inhibition - % -
CHEMBL3580936 Inhibition - % -
CHEMBL461609 HEYNEANINE Inhibition - % -
CHEMBL3580945 Inhibition - % -
CHEMBL3580946 Inhibition - % -