Assay Detail
Binding
CHEMBL3587049
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human human recombinant CP4H1 expressed in Escherichia coli Origami B(DE3) pre-incubated for 2 mins followed by alpha-ketoglutarate addition using dansylGlyProProGlyOEt substrate in Tris-HCl buffer at pH 7.8 at 30 degC by HPLC method
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Prolyl 4-hydroxylase subunit alpha-1 (CHEMBL1250350) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Selective inhibition of prolyl 4-hydroxylases by bipyridinedicarboxylates.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 3 | 5.61 | 6.08 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL332005 | — | — | 1 | 6.08 |
| CHEMBL3586252 | — | — | 1 | 5.92 |
| CHEMBL88972 | PYRIDINE-2,5-DICARBOXYLIC ACID | — | 1 | 4.82 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL332005 | — | IC50 | = | 840.0 | nM | 6.08 |
| CHEMBL3586252 | — | IC50 | = | 1200.0 | nM | 5.92 |
| CHEMBL88972 | PYRIDINE-2,5-DICARBOXYLIC ACID | IC50 | = | 15000.0 | nM | 4.82 |