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Assay Detail

CHEMBL3587908

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant PTP1B using p-NPP as substrate preincubated for 10 mins followed by substrate addition measured after 20 mins by microplate reader analysis
16
Total Activities
9
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein phosphatase non-receptor type 1 (CHEMBL335)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Selaginellin and biflavonoids as protein tyrosine phosphatase 1B inhibitors from Selaginella tamariscina and their glucose uptake stimulatory effects.
Nguyen PH, Ji DJ, Han YR, Choi JS, Rhyu DY, Min BS, Woo MH.
Bioorg Med Chem (2015) 23 : 3730 -3737
PubMed 25907369 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 5.17 5.47
Inhibition 7 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL169 URSOLIC ACID 2.0 1 5.47
CHEMBL515252 2 5.35
CHEMBL272861 TAIWANIAFLAVONE 2 5.27
CHEMBL63677 ROBUSTAFLAVONE 2 5.21
CHEMBL63354 AMENTOFLAVONE 2 5.13
CHEMBL1208973 CUPRESSUFLAVONE 2 5.02
CHEMBL3585680 2 5.01
CHEMBL3585679 2 4.88
CHEMBL426373 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL169 URSOLIC ACID IC50 = 3400.0 nM 5.47
CHEMBL515252 IC50 = 4500.0 nM 5.35
CHEMBL272861 TAIWANIAFLAVONE IC50 = 5400.0 nM 5.27
CHEMBL63677 ROBUSTAFLAVONE IC50 = 6200.0 nM 5.21
CHEMBL63354 AMENTOFLAVONE IC50 = 7400.0 nM 5.13
CHEMBL1208973 CUPRESSUFLAVONE IC50 = 9600.0 nM 5.02
CHEMBL3585680 IC50 = 9800.0 nM 5.01
CHEMBL3585679 IC50 = 13200.0 nM 4.88
CHEMBL426373 IC50 = 4500.0 nM -
CHEMBL63354 AMENTOFLAVONE Inhibition - % -
CHEMBL63677 ROBUSTAFLAVONE Inhibition - % -
CHEMBL1208973 CUPRESSUFLAVONE Inhibition - % -
CHEMBL272861 TAIWANIAFLAVONE Inhibition - % -
CHEMBL515252 Inhibition - % -
CHEMBL3585679 Inhibition - % -
CHEMBL3585680 Inhibition - % -