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Assay Detail

CHEMBL3587976

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Mixed-competitive inhibition of human recombinant PTP1B using p-NPP as substrate preincubated for 10 mins followed by substrate addition measured after 20 mins by Lineweaver-Burk/Dixon plot analysis
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein phosphatase non-receptor type 1 (CHEMBL335)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Selaginellin and biflavonoids as protein tyrosine phosphatase 1B inhibitors from Selaginella tamariscina and their glucose uptake stimulatory effects.
Nguyen PH, Ji DJ, Han YR, Choi JS, Rhyu DY, Min BS, Woo MH.
Bioorg Med Chem (2015) 23 : 3730 -3737
PubMed 25907369 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 6 5.17 5.35

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL272861 TAIWANIAFLAVONE 1 5.35
CHEMBL515252 1 5.34
CHEMBL169 URSOLIC ACID 2.0 1 5.19
CHEMBL63677 ROBUSTAFLAVONE 1 5.17
CHEMBL63354 AMENTOFLAVONE 1 5.11
CHEMBL3585679 1 4.84

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL272861 TAIWANIAFLAVONE Ki = 4500.0 nM 5.35
CHEMBL515252 Ki = 4600.0 nM 5.34
CHEMBL169 URSOLIC ACID Ki = 6400.0 nM 5.19
CHEMBL63677 ROBUSTAFLAVONE Ki = 6800.0 nM 5.17
CHEMBL63354 AMENTOFLAVONE Ki = 7800.0 nM 5.11
CHEMBL3585679 Ki = 14500.0 nM 4.84