Assay Detail
Binding
CHEMBL3588433
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Displacement of [3H]-PGE2 from human EP4 receptor overexpressed in human ECV304 cell membranes by scintillation proximity assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | ECV-304 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Prostaglandin E2 receptor EP4 subtype (CHEMBL1836) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
A selective prostaglandin E2 receptor subtype 2 (EP2) antagonist increases the macrophage-mediated clearance of amyloid-beta plaques.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 6 | 4.91 | 5.50 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3586353 | — | — | 1 | 5.50 |
| CHEMBL3586309 | — | — | 1 | 5.46 |
| CHEMBL3586359 | — | — | 1 | 4.75 |
| CHEMBL3586362 | — | — | 1 | 4.70 |
| CHEMBL3586361 | — | — | 1 | 4.52 |
| CHEMBL3586360 | — | — | 1 | 4.52 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3586353 | — | IC50 | = | 3200.0 | nM | 5.50 |
| CHEMBL3586309 | — | IC50 | = | 3500.0 | nM | 5.46 |
| CHEMBL3586359 | — | IC50 | = | 18000.0 | nM | 4.75 |
| CHEMBL3586362 | — | IC50 | = | 20000.0 | nM | 4.70 |
| CHEMBL3586361 | — | IC50 | = | 30000.0 | nM | 4.52 |
| CHEMBL3586360 | — | IC50 | = | 30000.0 | nM | 4.52 |