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Assay Detail

CHEMBL3591211

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of I-BET762 from 6His-Thr-BRD4 (1 to 477) BD1 (unknown origin) harboring BD2 Y390A mutant after 30 mins by TR-FRET analysis
34
Total Activities
30
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bromodomain-containing protein 4 (CHEMBL1163125)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-Based Optimization of Naphthyridones into Potent ATAD2 Bromodomain Inhibitors.
Bamborough P, Chung CW, Furze RC, Grandi P, Michon AM, Sheppard RJ, Barnett H, Diallo H, Dixon DP, Douault C, Jones EJ, Karamshi B, Mitchell DJ, Prinjha RK, Rau C, Watson RJ, Werner T, Demont EH.
J Med Chem (2015) 58 : 6151 -6178
PubMed 26230603 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 34 5.04 6.20

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3590391 1 6.20
CHEMBL3590389 1 5.80
CHEMBL3590405 2 5.40
CHEMBL3590388 2 5.40
CHEMBL3590397 1 5.30
CHEMBL3590404 1 5.30
CHEMBL3590399 1 5.20
CHEMBL3590380 1 5.20
CHEMBL3590396 2 5.20
CHEMBL3590402 1 5.20
CHEMBL3590401 1 5.20
CHEMBL3590385 1 5.10
CHEMBL3590384 1 5.10
CHEMBL3590382 1 5.00
CHEMBL3590407 1 5.00
CHEMBL3590381 1 5.00
CHEMBL3590403 1 5.00
CHEMBL3590394 2 5.00
CHEMBL3590392 1 5.00
CHEMBL3590387 1 4.90
CHEMBL3590398 1 4.90
CHEMBL3590395 1 4.90
CHEMBL3590393 1 4.90
CHEMBL3590390 1 4.90
CHEMBL3590383 1 4.80
CHEMBL3590406 1 4.60
CHEMBL3590400 1 4.60
CHEMBL3590379 1 4.60
CHEMBL3590386 1 4.30
CHEMBL3590408 1 4.10

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3590391 IC50 = 630.96 nM 6.20
CHEMBL3590389 IC50 = 1584.89 nM 5.80
CHEMBL3590405 IC50 = 3981.07 nM 5.40
CHEMBL3590405 IC50 = 3981.07 nM 5.40
CHEMBL3590388 IC50 = 3981.07 nM 5.40
CHEMBL3590388 IC50 = 5011.87 nM 5.30
CHEMBL3590397 IC50 = 5011.87 nM 5.30
CHEMBL3590404 IC50 = 5011.87 nM 5.30
CHEMBL3590399 IC50 = 6309.57 nM 5.20
CHEMBL3590380 IC50 = 6309.57 nM 5.20
CHEMBL3590396 IC50 = 6309.57 nM 5.20
CHEMBL3590401 IC50 = 6309.57 nM 5.20
CHEMBL3590402 IC50 = 6309.57 nM 5.20
CHEMBL3590385 IC50 = 7943.28 nM 5.10
CHEMBL3590384 IC50 = 7943.28 nM 5.10
CHEMBL3590394 IC50 = 10000.0 nM 5.00
CHEMBL3590403 IC50 = 10000.0 nM 5.00
CHEMBL3590381 IC50 = 10000.0 nM 5.00
CHEMBL3590407 IC50 = 10000.0 nM 5.00
CHEMBL3590382 IC50 = 10000.0 nM 5.00
CHEMBL3590392 IC50 = 10000.0 nM 5.00
CHEMBL3590396 IC50 = 12589.25 nM 4.90
CHEMBL3590398 IC50 = 12589.25 nM 4.90
CHEMBL3590387 IC50 = 12589.25 nM 4.90
CHEMBL3590395 IC50 = 12589.25 nM 4.90
CHEMBL3590393 IC50 = 12589.25 nM 4.90
CHEMBL3590390 IC50 = 12589.25 nM 4.90
CHEMBL3590394 IC50 = 15848.93 nM 4.80
CHEMBL3590383 IC50 = 15848.93 nM 4.80
CHEMBL3590406 IC50 = 25118.86 nM 4.60
CHEMBL3590400 IC50 = 25118.86 nM 4.60
CHEMBL3590379 IC50 = 25118.86 nM 4.60
CHEMBL3590386 IC50 = 50118.72 nM 4.30
CHEMBL3590408 IC50 = 79432.82 nM 4.10