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Assay Detail

CHEMBL3591483

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal GST tagged full-length human PRMT8 expressed in Escherichia coli (BL21(DE3) pre-incubated for 30 mins before addition of a [3H]SAM and peptide mix
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Protein arginine N-methyltransferase 8 (CHEMBL3108648)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Aryl Pyrazoles as Potent Inhibitors of Arginine Methyltransferases: Identification of the First PRMT6 Tool Compound.
Mitchell LH, Drew AE, Ribich SA, Rioux N, Swinger KK, Jacques SL, Lingaraj T, Boriack-Sjodin PA, Waters NJ, Wigle TJ, Moradei O, Jin L, Riera T, Porter-Scott M, Moyer MP, Smith JJ, Chesworth R, Copeland RA.
ACS Med Chem Lett (2015) 6 : 655 -659
PubMed 26101569 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 7.38 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3589029 1 8.70
CHEMBL3589030 1 8.15
CHEMBL3589026 1 8.00
CHEMBL3589028 1 8.00
CHEMBL3589031 1 8.00
CHEMBL3589027 1 7.85
CHEMBL3589037 1 7.68
CHEMBL3589033 1 7.66
CHEMBL3589032 1 7.66
CHEMBL3589036 1 7.64
CHEMBL3589913 1 7.55
CHEMBL3589038 1 7.36
CHEMBL3589034 1 7.26
CHEMBL3590417 1 7.19
CHEMBL3589912 1 7.17
CHEMBL3589035 1 7.16
CHEMBL3589042 1 6.75
CHEMBL3589039 1 6.65
CHEMBL3589043 1 6.46
CHEMBL3589040 1 6.35
CHEMBL3589041 1 5.82

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3589029 IC50 = 2.0 nM 8.70
CHEMBL3589030 IC50 = 7.0 nM 8.15
CHEMBL3589026 IC50 = 10.0 nM 8.00
CHEMBL3589028 IC50 = 10.0 nM 8.00
CHEMBL3589031 IC50 = 10.0 nM 8.00
CHEMBL3589027 IC50 = 14.0 nM 7.85
CHEMBL3589037 IC50 = 21.0 nM 7.68
CHEMBL3589033 IC50 = 22.0 nM 7.66
CHEMBL3589032 IC50 = 22.0 nM 7.66
CHEMBL3589036 IC50 = 23.0 nM 7.64
CHEMBL3589913 IC50 = 28.0 nM 7.55
CHEMBL3589038 IC50 = 44.0 nM 7.36
CHEMBL3589034 IC50 = 55.0 nM 7.26
CHEMBL3590417 IC50 = 65.0 nM 7.19
CHEMBL3589912 IC50 = 67.0 nM 7.17
CHEMBL3589035 IC50 = 70.0 nM 7.16
CHEMBL3589042 IC50 = 179.0 nM 6.75
CHEMBL3589039 IC50 = 223.0 nM 6.65
CHEMBL3589043 IC50 = 347.0 nM 6.46
CHEMBL3589040 IC50 = 450.0 nM 6.35
CHEMBL3589041 IC50 = 1500.0 nM 5.82