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Assay Detail

CHEMBL3592389

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of mouse recombinant iNOS using [3H]L-arginine substrate assessed as formation of [3H]L-citruline by liquid scintillation counting analysis
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nitric oxide synthase, inducible (CHEMBL3464)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Selective Acetamidine-Based Nitric Oxide Synthase Inhibitors: Synthesis, Docking, and Biological Studies.
Maccallini C, Montagnani M, Paciotti R, Ammazzalorso A, De Filippis B, Di Matteo M, Di Silvestre S, Fantacuzzi M, Giampietro L, Potenza MA, Re N, Pandolfi A, Amoroso R.
ACS Med Chem Lett (2015) 6 : 635 -640
PubMed 26101565 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 6.69 7.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3589092 1 7.00
CHEMBL107251 1400W 1 7.00
CHEMBL3589091 1 6.78
CHEMBL3589087 1 6.37
CHEMBL3589093 1 6.30
CHEMBL3589086 1 -
CHEMBL3589088 1 -
CHEMBL3589089 1 -
CHEMBL3589090 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3589092 IC50 = 100.0 nM 7.00
CHEMBL107251 1400W IC50 = 100.0 nM 7.00
CHEMBL3589091 IC50 = 165.0 nM 6.78
CHEMBL3589087 IC50 = 428.0 nM 6.37
CHEMBL3589093 IC50 = 500.0 nM 6.30
CHEMBL3589086 IC50 > 10000.0 nM -
CHEMBL3589088 IC50 > 10000.0 nM -
CHEMBL3589089 IC50 > 10000.0 nM -
CHEMBL3589090 IC50 > 10000.0 nM -