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Assay Detail

CHEMBL3594892

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of hedgehog signaling pathway in human HaCaT cells assessed as GLI1-mediated transcriptional activity after 12 hrs by luciferase assay
22
Total Activities
14
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HaCaT
Confidence 3 — Cell-line / Tissue
Curated By Autocuration

Publication

Hedgehog inhibitors from Artocarpus communis and Hyptis suaveolens.
Arai MA, Uchida K, Sadhu SK, Ahmed F, Koyano T, Kowithayakorn T, Ishibashi M.
Bioorg Med Chem (2015) 23 : 4150 -4154
PubMed 26178912 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 4.71 5.40
Inhibition 8 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL470287 2 5.40
CHEMBL471694 2 5.33
CHEMBL512307 2 5.12
CHEMBL374130 BONANNIONE A 2 4.84
CHEMBL469683 1 4.79
CHEMBL523392 2 4.63
CHEMBL3593940 1 4.53
CHEMBL3594223 1 4.40
CHEMBL3593935 2 4.40
CHEMBL3593939 2 4.39
CHEMBL3594224 1 4.36
CHEMBL3593938 2 4.36
CHEMBL3593937 1 -
CHEMBL3593936 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL470287 IC50 = 4000.0 nM 5.40
CHEMBL471694 IC50 = 4700.0 nM 5.33
CHEMBL512307 IC50 = 7600.0 nM 5.12
CHEMBL374130 BONANNIONE A IC50 = 14600.0 nM 4.84
CHEMBL469683 IC50 = 16300.0 nM 4.79
CHEMBL523392 IC50 = 23600.0 nM 4.63
CHEMBL3593940 IC50 = 29500.0 nM 4.53
CHEMBL3594223 IC50 = 39900.0 nM 4.40
CHEMBL3593935 IC50 = 40100.0 nM 4.40
CHEMBL3593939 IC50 = 40700.0 nM 4.39
CHEMBL3594224 IC50 = 43500.0 nM 4.36
CHEMBL3593938 IC50 = 44200.0 nM 4.36
CHEMBL471694 Inhibition - % -
CHEMBL470287 Inhibition - % -
CHEMBL512307 Inhibition - % -
CHEMBL374130 BONANNIONE A Inhibition - % -
CHEMBL3593939 Inhibition - % -
CHEMBL3593938 Inhibition - % -
CHEMBL3593935 Inhibition - % -
CHEMBL523392 Inhibition - % -
CHEMBL3593936 IC50 > 75000.0 nM -
CHEMBL3593937 IC50 > 75000.0 nM -