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Assay Detail

CHEMBL3606937

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant Escherichia coli MTAN expressed in Escherichia coli BL-21 DE3 using methylthioadenosine as substrate by xanthine oxidase coupling enzyme assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Escherichia coli
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (CHEMBL1250346)
Type SINGLE PROTEIN
Organism Escherichia coli O157:H7

Publication

Tight binding enantiomers of pre-clinical drug candidates.
Evans GB, Cameron SA, Luxenburger A, Guan R, Suarez J, Thomas K, Schramm VL, Tyler PC.
Bioorg Med Chem (2015) 23 : 5326 -5333
PubMed 26260335 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 2 9.34 10.32

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL405346 1 10.32
CHEMBL3604360 1 8.35

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL405346 Ki = 0.048 nM 10.32
CHEMBL3604360 Ki = 4.5 nM 8.35