Assay Detail
Binding
CHEMBL3607547
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Binding affinity to DAT in Sprague-Dawley rat brain synaptosomes assessed as induction of [3H]MPP+ release by liquid scintillation counting method
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Rattus norvegicus |
| Tissue | Brain |
| Subcellular | Synaptosomes |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Sodium-dependent dopamine transporter (CHEMBL338) ↗| Type | SINGLE PROTEIN |
| Organism | Rattus norvegicus |
Publication
Ethylenedioxy homologs of N-methyl-(3,4-methylenedioxyphenyl)-2-aminopropane (MDMA) and its corresponding cathinone analog methylenedioxymethcathinone: Interactions with transporters for serotonin, dopamine, and norepinephrine.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 6 | 6.32 | 7.12 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL43048 | MIDOMAFETAMINE | 3.0 | 1 | 7.12 |
| CHEMBL3604010 | METHYLONE | 2.0 | 1 | 6.88 |
| CHEMBL3603955 | — | — | 1 | 6.56 |
| CHEMBL3604009 | — | — | 1 | 6.30 |
| CHEMBL3603954 | — | — | 1 | 6.22 |
| CHEMBL3603956 | — | — | 1 | 4.84 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL43048 | MIDOMAFETAMINE | EC50 | = | 75.0 | nM | 7.12 |
| CHEMBL3604010 | METHYLONE | EC50 | = | 133.0 | nM | 6.88 |
| CHEMBL3603955 | — | EC50 | = | 276.0 | nM | 6.56 |
| CHEMBL3604009 | — | EC50 | = | 496.0 | nM | 6.30 |
| CHEMBL3603954 | — | EC50 | = | 597.0 | nM | 6.22 |
| CHEMBL3603956 | — | EC50 | = | 14600.0 | nM | 4.84 |