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Assay Detail

CHEMBL3608168

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of eIF4E-mediated translation in flexi rabbit reticulocyte lysate using luciferase mRNA preincubated for 60 mins before mRNA addition by luminescence based luciferase assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Oryctolagus cuniculus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Eukaryotic translation initiation factor 4E (CHEMBL5636)
Type SINGLE PROTEIN
Organism Oryctolagus cuniculus

Publication

Phosphate-modified analogues of m(7)GTP and m(7)Gppppm(7)G-Synthesis and biochemical properties.
Ziemniak M, Kowalska J, Lukaszewicz M, Zuberek J, Wnek K, Darzynkiewicz E, Jemielity J.
Bioorg Med Chem (2015) 23 : 5369 -5381
PubMed 26264844 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 5.58 6.25

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3604542 1 6.25
CHEMBL3604540 1 5.91
CHEMBL4299205 1 5.89
CHEMBL3604543 1 5.89
CHEMBL3604541 1 5.58
CHEMBL4299204 1 5.49
CHEMBL4299198 1 5.49
CHEMBL4299199 1 5.49
CHEMBL1094973 1 5.24
CHEMBL3351000 1 4.60
CHEMBL3604555 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3604542 IC50 = 560.0 nM 6.25
CHEMBL3604540 IC50 = 1240.0 nM 5.91
CHEMBL4299205 IC50 = 1280.0 nM 5.89
CHEMBL3604543 IC50 = 1300.0 nM 5.89
CHEMBL3604541 IC50 = 2610.0 nM 5.58
CHEMBL4299204 IC50 = 3260.0 nM 5.49
CHEMBL4299198 IC50 = 3220.0 nM 5.49
CHEMBL4299199 IC50 = 3260.0 nM 5.49
CHEMBL1094973 IC50 = 5720.0 nM 5.24
CHEMBL3351000 IC50 = 25000.0 nM 4.60
CHEMBL3604555 IC50 - -