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Assay Detail

CHEMBL3610436

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to BRD4 (unknown origin) expressed in Escherichia coli BL21 after 1 hr by proprietary competition assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bromodomain-containing protein 4 (CHEMBL1163125)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

BRD4 Structure-Activity Relationships of Dual PLK1 Kinase/BRD4 Bromodomain Inhibitor BI-2536.
Chen L, Yap JL, Yoshioka M, Lanning ME, Fountain RN, Raje M, Scheenstra JA, Strovel JW, Fletcher S.
ACS Med Chem Lett (2015) 6 : 764 -769
PubMed 26191363 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 18 6.82 8.06

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3609308 1 8.06
CHEMBL3609314 1 7.57
CHEMBL3609312 1 7.54
CHEMBL3609310 1 7.43
CHEMBL3609311 1 7.34
CHEMBL3609305 1 7.27
CHEMBL513909 BI-2536 2.0 1 7.25
CHEMBL3609309 1 7.22
CHEMBL3609313 1 7.00
CHEMBL3609315 1 7.00
CHEMBL3609300 1 6.33
CHEMBL3609307 1 6.01
CHEMBL3609304 1 5.85
CHEMBL3609301 1 5.35
CHEMBL3609299 1 5.10
CHEMBL3609306 1 -
CHEMBL3609303 1 -
CHEMBL3609302 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3609308 Ki = 8.7 nM 8.06
CHEMBL3609314 Ki = 27.0 nM 7.57
CHEMBL3609312 Ki = 29.0 nM 7.54
CHEMBL3609310 Ki = 37.0 nM 7.43
CHEMBL3609311 Ki = 46.0 nM 7.34
CHEMBL3609305 Ki = 54.0 nM 7.27
CHEMBL513909 BI-2536 Ki = 56.0 nM 7.25
CHEMBL3609309 Ki = 60.0 nM 7.22
CHEMBL3609313 Ki = 100.0 nM 7.00
CHEMBL3609315 Ki = 99.0 nM 7.00
CHEMBL3609300 Ki = 470.0 nM 6.33
CHEMBL3609307 Ki = 970.0 nM 6.01
CHEMBL3609304 Ki = 1400.0 nM 5.85
CHEMBL3609301 Ki = 4500.0 nM 5.35
CHEMBL3609299 Ki = 7950.0 nM 5.10
CHEMBL3609306 Ki > 10000.0 nM -
CHEMBL3609303 Ki > 10000.0 nM -
CHEMBL3609302 Ki > 10000.0 nM -