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Assay Detail

CHEMBL3610609

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PI3Kalpha (unknown origin) using phosphatidylinositol as substrate preincubated for 15 mins followed by ATP addition measured after 1 hr by KinaseGlo luminescence assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Identification and optimisation of 4,5-dihydrobenzo[1,2-d:3,4-d]bisthiazole and 4,5-dihydrothiazolo[4,5-h]quinazoline series of selective phosphatidylinositol-3 kinase alpha inhibitors.
Fairhurst RA, Gerspacher M, Imbach-Weese P, Mah R, Caravatti G, Furet P, Fritsch C, Schnell C, Blanz J, Blasco F, Desrayaud S, Guthy DA, Knapp M, Arz D, Wirth J, Roehn-Carnemolla E, Luu VH.
Bioorg Med Chem Lett (2015) 25 : 3575 -3581
PubMed 26199119 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 8.06 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3609522 1 8.40
CHEMBL3609529 1 8.40
CHEMBL3609527 1 8.30
CHEMBL3609526 1 8.30
CHEMBL3609523 1 8.30
CHEMBL3609525 1 8.22
CHEMBL3609524 1 8.22
CHEMBL2397186 1 8.15
CHEMBL3609516 1 8.10
CHEMBL3609520 1 8.05
CHEMBL3609517 1 8.05
CHEMBL3609521 1 8.00
CHEMBL3609519 1 7.96
CHEMBL3609514 1 7.89
CHEMBL3609518 1 7.89
CHEMBL3609528 1 7.85
CHEMBL3218581 1 7.82
CHEMBL3609515 1 7.16

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3609522 IC50 = 4.0 nM 8.40
CHEMBL3609529 IC50 = 4.0 nM 8.40
CHEMBL3609527 IC50 = 5.0 nM 8.30
CHEMBL3609526 IC50 = 5.0 nM 8.30
CHEMBL3609523 IC50 = 5.0 nM 8.30
CHEMBL3609525 IC50 = 6.0 nM 8.22
CHEMBL3609524 IC50 = 6.0 nM 8.22
CHEMBL2397186 IC50 = 7.0 nM 8.15
CHEMBL3609516 IC50 = 8.0 nM 8.10
CHEMBL3609520 IC50 = 9.0 nM 8.05
CHEMBL3609517 IC50 = 9.0 nM 8.05
CHEMBL3609521 IC50 = 10.0 nM 8.00
CHEMBL3609519 IC50 = 11.0 nM 7.96
CHEMBL3609514 IC50 = 13.0 nM 7.89
CHEMBL3609518 IC50 = 13.0 nM 7.89
CHEMBL3609528 IC50 = 14.0 nM 7.85
CHEMBL3218581 IC50 = 15.0 nM 7.82
CHEMBL3609515 IC50 = 70.0 nM 7.16