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Assay Detail

CHEMBL3620254

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to Lymnaea stagnalis AChBP after 300 seconds by SPR biosensor analysis
8
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Lymnaea stagnalis
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Acetylcholine-binding protein (CHEMBL6084)
Type SINGLE PROTEIN
Organism Lymnaea stagnalis

Publication

Exploration of the molecular architecture of the orthosteric binding site in the α4β2 nicotinic acetylcholine receptor with analogs of 3-(dimethylamino)butyl dimethylcarbamate (DMABC) and 1-(pyridin-3-yl)-1,4-diazepane.
Bach TB, Jensen AA, Petersen JG, Sørensen TE, Della Volpe S, Liu J, Blaazer AR, van Muijlwijk-Koezen JE, Balle T, Frølund B.
Eur J Med Chem (2015) 102 : 425 -444
PubMed 26301559 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 8 6.80 7.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3616577 2 7.70
CHEMBL3616575 2 6.80
CHEMBL3 NICOTINE 4.0 2 6.50
CHEMBL122270 LOBELINE 2.0 2 6.20

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3616577 Kd = 20.0 nM 7.70
CHEMBL3616577 Kd = 19.95 nM 7.70
CHEMBL3616575 Kd = 160.0 nM 6.80
CHEMBL3616575 Kd = 158.49 nM 6.80
CHEMBL3 NICOTINE Kd = 320.0 nM 6.50
CHEMBL3 NICOTINE Kd = 316.23 nM 6.50
CHEMBL122270 LOBELINE Kd = 630.0 nM 6.20
CHEMBL122270 LOBELINE Kd = 630.96 nM 6.20