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Assay Detail

CHEMBL3624698

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of KDM2A (unknown origin) using Biotin-H3(28-48)K36Me2 and H3(28-48)K36Me2 substrates incubated for 30 mins by alpha screen assay
32
Total Activities
32
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Lysine-specific demethylase 2A (CHEMBL1938210)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Optimisation of a triazolopyridine based histone demethylase inhibitor yields a potent and selective KDM2A (FBXL11) inhibitor.
England KS, Tumber A, Krojer T, Scozzafava G, Ng SS, Daniel M, Szykowska A, Che K, von Delft F, Burgess-Brown NA, Kawamura A, Schofield CJ, Brennan PE.
Medchemcomm (2014) 5 : 1879 -1886
PubMed 26682034 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 31 5.34 7.20
pIC50 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3621881 1 7.20
CHEMBL3621878 1 6.90
CHEMBL3621872 1 6.40
CHEMBL3621880 1 6.20
CHEMBL3621873 1 6.00
CHEMBL3621879 1 5.70
CHEMBL3621864 1 5.60
CHEMBL3621882 1 5.50
CHEMBL3621863 1 5.40
CHEMBL3621852 1 5.40
CHEMBL3621877 1 5.30
CHEMBL3621851 1 5.30
CHEMBL3621868 1 5.30
CHEMBL3621858 1 5.20
CHEMBL3621860 1 5.20
CHEMBL3621855 1 5.20
CHEMBL3621869 1 5.20
CHEMBL3621876 1 5.10
CHEMBL3621857 1 5.10
CHEMBL3621859 1 5.10
CHEMBL3621861 1 5.10
CHEMBL3621862 1 5.10
CHEMBL3621870 1 5.00
CHEMBL3621856 1 5.00
CHEMBL3621853 1 4.90
CHEMBL3621875 1 4.90
CHEMBL3621871 1 4.90
CHEMBL3621854 1 4.50
CHEMBL3621867 1 4.30
CHEMBL3621866 1 4.10

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3621881 IC50 = 63.1 nM 7.20
CHEMBL3621878 IC50 = 125.89 nM 6.90
CHEMBL3621872 IC50 = 398.11 nM 6.40
CHEMBL3621880 IC50 = 630.96 nM 6.20
CHEMBL3621873 IC50 = 1000.0 nM 6.00
CHEMBL3621879 IC50 = 1995.26 nM 5.70
CHEMBL3621864 IC50 = 2511.89 nM 5.60
CHEMBL3621882 IC50 = 3162.28 nM 5.50
CHEMBL3621863 IC50 = 3981.07 nM 5.40
CHEMBL3621852 IC50 = 3981.07 nM 5.40
CHEMBL3621877 IC50 = 5011.87 nM 5.30
CHEMBL3621851 IC50 = 5011.87 nM 5.30
CHEMBL3621868 IC50 = 5011.87 nM 5.30
CHEMBL3621860 IC50 = 6309.57 nM 5.20
CHEMBL3621858 IC50 = 6309.57 nM 5.20
CHEMBL3621869 IC50 = 6309.57 nM 5.20
CHEMBL3621855 IC50 = 6309.57 nM 5.20
CHEMBL3621876 IC50 = 7943.28 nM 5.10
CHEMBL3621857 IC50 = 7943.28 nM 5.10
CHEMBL3621859 IC50 = 7943.28 nM 5.10
CHEMBL3621861 IC50 = 7943.28 nM 5.10
CHEMBL3621862 IC50 = 7943.28 nM 5.10
CHEMBL3621856 IC50 = 10000.0 nM 5.00
CHEMBL3621870 IC50 = 10000.0 nM 5.00
CHEMBL3621853 IC50 = 12589.25 nM 4.90
CHEMBL3621875 IC50 = 12589.25 nM 4.90
CHEMBL3621871 IC50 = 12589.25 nM 4.90
CHEMBL3621854 IC50 = 31622.78 nM 4.50
CHEMBL3621867 IC50 = 50118.72 nM 4.30
CHEMBL3621866 IC50 = 79432.82 nM 4.10
CHEMBL3621865 IC50 > 100000.0 nM -
CHEMBL3621874 pIC50 - -