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Assay Detail

CHEMBL3626889

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human KAT2B catalytic domain using biotinylated histone H3 (1 to 21 amino acid residues) as substrate preincubated for 10 mins followed by acetyl-CoA addition measured after 10 mins by time resolved fluorescence assay
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone acetyltransferase KAT2B (CHEMBL5500)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Pyrido- and benzisothiazolones as inhibitors of histone acetyltransferases (HATs)
Furdas SD, Hoffmann I, Robaa D, Herquel B, Malinka W, Swiatek P, Akhtar A, Sippl W, Jung M
Medchemcomm (2014) 5 : 1856 -1862
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 5.02 5.45

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL318367 1 5.45
CHEMBL1732009 1 5.34
CHEMBL1795608 1 5.28
CHEMBL3621679 1 5.24
CHEMBL1391552 1 5.24
CHEMBL1733695 1 5.22
CHEMBL1392315 1 5.21
CHEMBL1795613 1 5.18
CHEMBL1795619 1 5.16
CHEMBL1720939 1 5.16
CHEMBL1830867 1 5.03
CHEMBL2087789 1 5.03
CHEMBL106011 1 5.01
CHEMBL3621676 1 4.97
CHEMBL3621677 1 4.93
CHEMBL608699 1 4.87
CHEMBL3621672 1 4.87
CHEMBL3621678 1 4.82
CHEMBL1795618 1 4.76
CHEMBL3621675 1 4.38
CHEMBL3621673 1 4.17
CHEMBL1795624 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL318367 IC50 = 3530.0 nM 5.45
CHEMBL1732009 IC50 = 4550.0 nM 5.34
CHEMBL1795608 IC50 = 5300.0 nM 5.28
CHEMBL3621679 IC50 = 5820.0 nM 5.24
CHEMBL1391552 IC50 = 5770.0 nM 5.24
CHEMBL1733695 IC50 = 6010.0 nM 5.22
CHEMBL1392315 IC50 = 6190.0 nM 5.21
CHEMBL1795613 IC50 = 6640.0 nM 5.18
CHEMBL1795619 IC50 = 6940.0 nM 5.16
CHEMBL1720939 IC50 = 6950.0 nM 5.16
CHEMBL1830867 IC50 = 9410.0 nM 5.03
CHEMBL2087789 IC50 = 9300.0 nM 5.03
CHEMBL106011 IC50 = 9740.0 nM 5.01
CHEMBL3621676 IC50 = 10700.0 nM 4.97
CHEMBL3621677 IC50 = 11800.0 nM 4.93
CHEMBL608699 IC50 = 13600.0 nM 4.87
CHEMBL3621672 IC50 = 13600.0 nM 4.87
CHEMBL3621678 IC50 = 15100.0 nM 4.82
CHEMBL1795618 IC50 = 17450.0 nM 4.76
CHEMBL3621675 IC50 = 41800.0 nM 4.38
CHEMBL3621673 IC50 = 67000.0 nM 4.17
CHEMBL1795624 IC50 = 130000.0 nM -