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Assay Detail

CHEMBL3631861

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of 5-LO in mouse BMM cells assessed as formation of LTC4 after 30 mins by enzyme immunoassay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type BMM
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Polyunsaturated fatty acid 5-lipoxygenase (CHEMBL5211)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

5-Lipoxygenase inhibitors suppress RANKL-induced osteoclast formation via NFATc1 expression.
Kang JH, Ting Z, Moon MR, Sim JS, Lee JM, Doh KE, Hong S, Cui M, Choi S, Chang HW, Park Choo HY, Yim M.
Bioorg Med Chem (2015) 23 : 7069 -7078
PubMed 26432605 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 5.80 6.72

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL93 ZILEUTON 4.0 1 6.72
CHEMBL3629175 1 5.76
CHEMBL3629176 1 5.39
CHEMBL3629177 1 5.31

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL93 ZILEUTON IC50 = 190.0 nM 6.72
CHEMBL3629175 IC50 = 1720.0 nM 5.76
CHEMBL3629176 IC50 = 4100.0 nM 5.39
CHEMBL3629177 IC50 = 4900.0 nM 5.31