Assay Detail
Binding
CHEMBL3632106
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Displacement of [3H]-1alpha,25-dihydroxyvitamin D3 from recombinant human VDR LBD expressed in Escherichia coli BL21 (DE3) pLysS after 16 hrs
5
Total Activities
5
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Fine tuning of agonistic/antagonistic activity for vitamin D receptor by 22-alkyl chain length of ligands: 22S-Hexyl compound unexpectedly restored agonistic activity.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Inhibition | 3 | - | - |
| IC50 | 2 | 9.95 | 10.22 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL846 | CALCITRIOL | 4.0 | 1 | 10.22 |
| CHEMBL3629552 | — | — | 1 | 9.68 |
| CHEMBL1214509 | — | — | 1 | - |
| CHEMBL1214508 | — | — | 1 | - |
| CHEMBL1212956 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL846 | CALCITRIOL | IC50 | = | 0.06 | nM | 10.22 |
| CHEMBL3629552 | — | IC50 | = | 0.21 | nM | 9.68 |
| CHEMBL1214509 | — | Inhibition | - | % | - | |
| CHEMBL1214508 | — | Inhibition | - | % | - | |
| CHEMBL1212956 | — | Inhibition | - | % | - |