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Assay Detail

CHEMBL3636231

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity against NR1-1a/NR2A NMDA receptor (unknown origin) expressed in mouse recombinant L(tk-) cells assessed as inhibition of glycine and glutamate induced excitotoxicity incubated for 12 hrs by LDH assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Glutamate NMDA receptor; GRIN1/GRIN2A (CHEMBL1907604)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Novel Potent N-Methyl-d-aspartate (NMDA) Receptor Antagonists or σ1 Receptor Ligands Based on Properly Substituted 1,4-Dioxane Ring.
Bonifazi A, Del Bello F, Mammoli V, Piergentili A, Petrelli R, Cimarelli C, Pellei M, Schepmann D, Wünsch B, Barocelli E, Bertoni S, Flammini L, Amantini C, Nabissi M, Santoni G, Vistoli G, Quaglia W.
J Med Chem (2015) 58 : 8601 -8615
PubMed 26430967 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 7.73 8.01

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1165411 DEXOXADROL 2.0 1 8.01
CHEMBL395091 ESKETAMINE 4.0 1 7.94
CHEMBL275528 PHENCYCLIDINE 2.0 1 7.93
CHEMBL3633211 1 7.75
CHEMBL3633387 1 7.62
CHEMBL3633210 1 7.15

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1165411 DEXOXADROL IC50 = 9.7 nM 8.01
CHEMBL395091 ESKETAMINE IC50 = 11.4 nM 7.94
CHEMBL275528 PHENCYCLIDINE IC50 = 11.8 nM 7.93
CHEMBL3633211 IC50 = 17.7 nM 7.75
CHEMBL3633387 IC50 = 24.2 nM 7.62
CHEMBL3633210 IC50 = 71.2 nM 7.15