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Assay Detail

CHEMBL3638153

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Functional
Inhibition of Cav1.2 calcium current measured using whole cell patch clamp in guinea pig atrial myocytes
1
Total Activities
1
Compounds Tested
1
Activity Types
6
Assay Parameters

Assay Information

Assay Type Functional
Organism Cavia porcellus
Tissue Heart
Cell Type Cardiac Muscle Cell
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

IC50 data for the L-type calcium channel extracted from a set of literature articles

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 6.55 6.55

Assay Parameters

Parameter Type Value Units Text
CA_SOL_CONC CA_SOL_CONC 2.0 mM
DETECTION_METHOD DETECTION_METHOD - Whole cell patch clamp
HOLDING_POTENTIAL HOLDING_POTENTIAL - -40
MEAS_VOLT MEAS_VOLT 10.0 mV
T1_PULSE T1_PULSE 0.2 s
TEMP TEMP - 22 pm 1

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL441 THIOPENTAL 4.0 1 6.55

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL441 THIOPENTAL IC50 = 280.0 nM 6.55