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Assay Detail

CHEMBL3705076

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Enzyme Inhibition Assay: The inhibition of PI3Kβ, PI3Kα, PI3Kγ and PI3Kδ was evaluated in a Kinase Glo based enzyme activity assay using human recombinant enzymes. The assay measures depletion of ATP after incubation with enzyme, PIP2 and ATP plus compound. ATP at the end of the reaction is detected by addition of Kinase Glo reagent, in this the Ultra Glo luciferase (Promega) uses the ATP as a substrate to catalyze the mono-oxygenation of luciferin and the generation of light. A direct relationship exists between the luminescence measured with the Kinase-Glo Plus Reagent and the amount of ATP remaining in a completed kinase reaction and luminescence is inversely related to kinase activity.
102
Total Activities
93
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Chromenone derivatives
(2014)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 102 8.12 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3656387 1 9.00
CHEMBL3656388 1 8.70
CHEMBL3656386 1 8.70
CHEMBL3408258 1 8.70
CHEMBL3656402 2 8.70
CHEMBL3656441 1 8.52
CHEMBL3408266 1 8.52
CHEMBL3656404 2 8.52
CHEMBL3656448 1 8.40
CHEMBL3408245 1 8.40
CHEMBL3656432 2 8.40
CHEMBL3408261 1 8.40
CHEMBL3408263 1 8.40
CHEMBL3656428 1 8.40
CHEMBL3656429 1 8.40
CHEMBL3408260 1 8.40
CHEMBL3656431 1 8.40
CHEMBL3408259 1 8.40
CHEMBL3656443 1 8.40
CHEMBL3656410 1 8.40
CHEMBL3656435 1 8.40
CHEMBL3408269 1 8.40
CHEMBL3656420 1 8.40
CHEMBL3408254 1 8.40
CHEMBL3656416 1 8.40
CHEMBL3656437 1 8.40
CHEMBL3656414 1 8.40
CHEMBL3656440 1 8.40
CHEMBL3639553 1 8.40
CHEMBL3656433 1 8.40

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3656387 IC50 = 1.0 nM 9.00
CHEMBL3656388 IC50 = 2.0 nM 8.70
CHEMBL3656386 IC50 = 2.0 nM 8.70
CHEMBL3408258 IC50 = 2.0 nM 8.70
CHEMBL3656402 IC50 = 2.0 nM 8.70
CHEMBL3408266 IC50 = 3.0 nM 8.52
CHEMBL3656441 IC50 = 3.0 nM 8.52
CHEMBL3656404 IC50 = 3.0 nM 8.52
CHEMBL3656410 IC50 = 4.0 nM 8.40
CHEMBL3656429 IC50 = 4.0 nM 8.40
CHEMBL3408261 IC50 = 4.0 nM 8.40
CHEMBL3408263 IC50 = 4.0 nM 8.40
CHEMBL3656404 IC50 = 4.0 nM 8.40
CHEMBL3656402 IC50 = 4.0 nM 8.40
CHEMBL3656392 IC50 = 4.0 nM 8.40
CHEMBL3408246 IC50 = 4.0 nM 8.40
CHEMBL3408245 IC50 = 4.0 nM 8.40
CHEMBL3656428 IC50 = 4.0 nM 8.40
CHEMBL3408260 IC50 = 4.0 nM 8.40
CHEMBL3656431 IC50 = 4.0 nM 8.40
CHEMBL3656432 IC50 = 4.0 nM 8.40
CHEMBL3656448 IC50 = 4.0 nM 8.40
CHEMBL3408259 IC50 = 4.0 nM 8.40
CHEMBL3656443 IC50 = 4.0 nM 8.40
CHEMBL3656435 IC50 = 4.0 nM 8.40
CHEMBL3408269 IC50 = 4.0 nM 8.40
CHEMBL3656437 IC50 = 4.0 nM 8.40
CHEMBL3656420 IC50 = 4.0 nM 8.40
CHEMBL3656440 IC50 = 4.0 nM 8.40
CHEMBL3656433 IC50 = 4.0 nM 8.40
CHEMBL3408254 IC50 = 4.0 nM 8.40
CHEMBL3656416 IC50 = 4.0 nM 8.40
CHEMBL3639553 IC50 = 4.0 nM 8.40
CHEMBL3656414 IC50 = 4.0 nM 8.40
CHEMBL3656447 IC50 = 5.0 nM 8.30
CHEMBL3408264 IC50 = 5.0 nM 8.30
CHEMBL3408276 IC50 = 5.0 nM 8.30
CHEMBL3656417 IC50 = 5.0 nM 8.30
CHEMBL3656407 IC50 = 5.0 nM 8.30
CHEMBL3408253 IC50 = 5.0 nM 8.30
CHEMBL3407508 IC50 = 5.0 nM 8.30
CHEMBL3656408 IC50 = 5.0 nM 8.30
CHEMBL3656405 IC50 = 5.0 nM 8.30
CHEMBL3408255 IC50 = 5.0 nM 8.30
CHEMBL3656432 IC50 = 5.0 nM 8.30
CHEMBL3656439 IC50 = 5.0 nM 8.30
CHEMBL3408268 IC50 = 5.0 nM 8.30
CHEMBL3408265 IC50 = 5.0 nM 8.30
CHEMBL3656393 IC50 = 5.0 nM 8.30
CHEMBL3895589 IC50 = 5.0 nM 8.30