Assay Detail
Binding
CHEMBL3705108
Review assay metadata, readout intent, target linkage, and publication context from the same page.
ALDH2 Assay: Standard ALDH2 reaction mixtures contained 150 uM formaldehyde, 2.5 mM NAD+, 10 mM MgCl2 and 10 nM recombinant human ALDH2 in 50 mM Hepes buffer, pH 7.4, 0.01% Tween 20 in a final volume of 50 ul using 384-well plates. After 60 min of pre-incubation of compound with ALDH2 and formaldehyde, the reaction was started by adding NAD+ and the reaction mixture was allowed to proceed for 90 minutes. Activity of the enzyme was determined by monitoring NADH formation using Perkin-Elmer Envision Reader with excitation and emission wavelengths set at 340 and 460 nm, respectively.
41
Total Activities
41
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Aldehyde dehydrogenase, mitochondrial (CHEMBL1935) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
ALDH-2 inhibitors in the treatment of addiction
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 41 | 7.51 | 8.64 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3660752 | — | — | 1 | 8.64 |
| CHEMBL3660748 | — | — | 1 | 8.40 |
| CHEMBL3660751 | — | — | 1 | 8.30 |
| CHEMBL3660747 | — | — | 1 | 8.30 |
| CHEMBL3660742 | — | — | 1 | 8.30 |
| CHEMBL3660728 | — | — | 1 | 8.30 |
| CHEMBL5856466 | — | — | 1 | 8.22 |
| CHEMBL3660749 | — | — | 1 | 8.22 |
| CHEMBL3660744 | — | — | 1 | 8.22 |
| CHEMBL3660739 | — | — | 1 | 8.22 |
| CHEMBL3660730 | — | — | 1 | 8.19 |
| CHEMBL3660732 | — | — | 1 | 8.15 |
| CHEMBL3660738 | — | — | 1 | 8.10 |
| CHEMBL3660735 | — | — | 1 | 8.05 |
| CHEMBL3660727 | — | — | 1 | 8.05 |
| CHEMBL3660726 | — | — | 1 | 8.05 |
| CHEMBL3660745 | — | — | 1 | 7.82 |
| CHEMBL3660731 | — | — | 1 | 7.82 |
| CHEMBL3660741 | — | — | 1 | 7.82 |
| CHEMBL3660743 | — | — | 1 | 7.80 |
| CHEMBL3660722 | — | — | 1 | 7.68 |
| CHEMBL3660746 | — | — | 1 | 7.46 |
| CHEMBL3660736 | — | — | 1 | 7.37 |
| CHEMBL3660716 | — | — | 1 | 7.33 |
| CHEMBL3660712 | — | — | 1 | 7.24 |
| CHEMBL3660721 | — | — | 1 | 7.24 |
| CHEMBL3660740 | — | — | 1 | 7.19 |
| CHEMBL3660725 | — | — | 1 | 7.07 |
| CHEMBL3660719 | — | — | 1 | 7.05 |
| CHEMBL3660733 | — | — | 1 | 7.02 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3660752 | — | IC50 | = | 2.3 | nM | 8.64 |
| CHEMBL3660748 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL3660747 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL3660742 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL3660728 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL3660751 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL5856466 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL3660749 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL3660744 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL3660739 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL3660730 | — | IC50 | = | 6.5 | nM | 8.19 |
| CHEMBL3660732 | — | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL3660738 | — | IC50 | = | 8.0 | nM | 8.10 |
| CHEMBL3660726 | — | IC50 | = | 9.0 | nM | 8.05 |
| CHEMBL3660727 | — | IC50 | = | 9.0 | nM | 8.05 |
| CHEMBL3660735 | — | IC50 | = | 9.0 | nM | 8.05 |
| CHEMBL3660745 | — | IC50 | = | 15.0 | nM | 7.82 |
| CHEMBL3660731 | — | IC50 | = | 15.0 | nM | 7.82 |
| CHEMBL3660741 | — | IC50 | = | 15.0 | nM | 7.82 |
| CHEMBL3660743 | — | IC50 | = | 16.0 | nM | 7.80 |
| CHEMBL3660722 | — | IC50 | = | 21.0 | nM | 7.68 |
| CHEMBL3660746 | — | IC50 | = | 35.0 | nM | 7.46 |
| CHEMBL3660736 | — | IC50 | = | 43.0 | nM | 7.37 |
| CHEMBL3660716 | — | IC50 | = | 47.0 | nM | 7.33 |
| CHEMBL3660712 | — | IC50 | = | 57.7 | nM | 7.24 |
| CHEMBL3660721 | — | IC50 | = | 57.0 | nM | 7.24 |
| CHEMBL3660740 | — | IC50 | = | 64.0 | nM | 7.19 |
| CHEMBL3660725 | — | IC50 | = | 86.0 | nM | 7.07 |
| CHEMBL3660719 | — | IC50 | = | 90.0 | nM | 7.05 |
| CHEMBL3660733 | — | IC50 | = | 95.0 | nM | 7.02 |
| CHEMBL3660723 | — | IC50 | = | 96.0 | nM | 7.02 |
| CHEMBL3915386 | — | IC50 | = | 109.0 | nM | 6.96 |
| CHEMBL3660724 | — | IC50 | = | 156.0 | nM | 6.81 |
| CHEMBL3660729 | — | IC50 | = | 174.0 | nM | 6.76 |
| CHEMBL3660734 | — | IC50 | = | 266.0 | nM | 6.58 |
| CHEMBL3660713 | — | IC50 | = | 329.0 | nM | 6.48 |
| CHEMBL3918259 | — | IC50 | = | 386.0 | nM | 6.41 |
| CHEMBL3660718 | — | IC50 | = | 528.0 | nM | 6.28 |
| CHEMBL3660717 | — | IC50 | = | 1706.0 | nM | 5.77 |
| CHEMBL3660714 | — | IC50 | = | 2181.0 | nM | 5.66 |
| CHEMBL3935026 | — | IC50 | < | 4.9 | nM | - |