Assay Detail
Binding
CHEMBL3705746
Review assay metadata, readout intent, target linkage, and publication context from the same page.
In Vitro Inhibition Assay: An in vitro assay showed inhibition of CXCR2-mediated intracellular calcium release. Briefly, human neutrophils were suspended in HBSS- (without Ca2+ and Mg2+) containing 10 mM HEPES and FLIPR Calcium 3 dye (3.1x107 cells in total volume 1.7 mL). Cells were aliquoted (200 uL of the cell suspension per tube, 8 tubes total) and 2 uL of the designated compound (with appropriate dilutions) were added to each of 6 tubes. As controls, 2 uL of DMSO (1% final concentration) were added to 2 other tubes. Cells were incubated for 30 min at 37 C. After dye loading, tubes were centrifuged at 6,000 rpm for 1 min, supernatant was removed and the cell pellet was re-suspended in 200 uL of HBSS+ (with Ca2+ and Mg2+) containing 10 mM HEPES. The test compound or DMSO (control) was added again at the same concentrations that were used during cell loading. The cell suspension was aliquoted into a 96-well Reading Plate (Corning) in a volume of 90 uL (105 cells/well).
177
Total Activities
177
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Pyrimidinecarboxamides as CXCR2 modulators
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 177 | 6.37 | 7.85 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3658321 | — | — | 1 | 7.85 |
| CHEMBL3658278 | — | — | 1 | 7.80 |
| CHEMBL3426944 | — | — | 1 | 7.66 |
| CHEMBL3654441 | — | — | 1 | 7.55 |
| CHEMBL3658341 | — | — | 1 | 7.50 |
| CHEMBL3658338 | — | — | 1 | 7.43 |
| CHEMBL3342269 | — | — | 1 | 7.42 |
| CHEMBL3426949 | — | — | 1 | 7.28 |
| CHEMBL3654444 | — | — | 1 | 7.23 |
| CHEMBL3654446 | — | — | 1 | 7.14 |
| CHEMBL3342320 | — | — | 1 | 7.11 |
| CHEMBL3426945 | — | — | 1 | 7.10 |
| CHEMBL3639571 | — | — | 1 | 7.09 |
| CHEMBL3342318 | — | — | 1 | 6.98 |
| CHEMBL3658241 | — | — | 1 | 6.95 |
| CHEMBL3658340 | — | — | 1 | 6.94 |
| CHEMBL3658343 | — | — | 1 | 6.78 |
| CHEMBL3342312 | — | — | 1 | 6.76 |
| CHEMBL3658323 | — | — | 1 | 6.71 |
| CHEMBL3658274 | — | — | 1 | 6.68 |
| CHEMBL3658260 | — | — | 1 | 6.67 |
| CHEMBL3342304 | — | — | 1 | 6.56 |
| CHEMBL3342313 | — | — | 1 | 6.56 |
| CHEMBL3342314 | — | — | 1 | 6.56 |
| CHEMBL3342319 | — | — | 1 | 6.53 |
| CHEMBL3658245 | — | — | 1 | 6.46 |
| CHEMBL3426951 | — | — | 1 | 6.41 |
| CHEMBL3342291 | — | — | 1 | 6.41 |
| CHEMBL3658335 | — | — | 1 | 6.28 |
| CHEMBL3342317 | — | — | 1 | 6.28 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3658321 | — | IC50 | = | 14.0 | nM | 7.85 |
| CHEMBL3658278 | — | IC50 | = | 16.0 | nM | 7.80 |
| CHEMBL3426944 | — | IC50 | = | 22.0 | nM | 7.66 |
| CHEMBL3654441 | — | IC50 | = | 28.0 | nM | 7.55 |
| CHEMBL3658341 | — | IC50 | = | 32.0 | nM | 7.50 |
| CHEMBL3658338 | — | IC50 | = | 37.0 | nM | 7.43 |
| CHEMBL3342269 | — | IC50 | = | 38.0 | nM | 7.42 |
| CHEMBL3426949 | — | IC50 | = | 52.0 | nM | 7.28 |
| CHEMBL3654444 | — | IC50 | = | 59.0 | nM | 7.23 |
| CHEMBL3654446 | — | IC50 | = | 72.0 | nM | 7.14 |
| CHEMBL3342320 | — | IC50 | = | 78.0 | nM | 7.11 |
| CHEMBL3426945 | — | IC50 | = | 79.0 | nM | 7.10 |
| CHEMBL3639571 | — | IC50 | = | 81.0 | nM | 7.09 |
| CHEMBL3342318 | — | IC50 | = | 105.0 | nM | 6.98 |
| CHEMBL3658241 | — | IC50 | = | 112.0 | nM | 6.95 |
| CHEMBL3658340 | — | IC50 | = | 116.0 | nM | 6.94 |
| CHEMBL3658343 | — | IC50 | = | 166.0 | nM | 6.78 |
| CHEMBL3342312 | — | IC50 | = | 173.0 | nM | 6.76 |
| CHEMBL3658323 | — | IC50 | = | 194.0 | nM | 6.71 |
| CHEMBL3658274 | — | IC50 | = | 210.0 | nM | 6.68 |
| CHEMBL3658260 | — | IC50 | = | 216.0 | nM | 6.67 |
| CHEMBL3342304 | — | IC50 | = | 277.0 | nM | 6.56 |
| CHEMBL3342313 | — | IC50 | = | 275.0 | nM | 6.56 |
| CHEMBL3342314 | — | IC50 | = | 278.0 | nM | 6.56 |
| CHEMBL3342319 | — | IC50 | = | 294.0 | nM | 6.53 |
| CHEMBL3658245 | — | IC50 | = | 350.0 | nM | 6.46 |
| CHEMBL3342291 | — | IC50 | = | 393.0 | nM | 6.41 |
| CHEMBL3426951 | — | IC50 | = | 387.0 | nM | 6.41 |
| CHEMBL3342317 | — | IC50 | = | 520.0 | nM | 6.28 |
| CHEMBL3658335 | — | IC50 | = | 529.0 | nM | 6.28 |
| CHEMBL3342282 | — | IC50 | = | 542.0 | nM | 6.27 |
| CHEMBL3654443 | — | IC50 | = | 611.0 | nM | 6.21 |
| CHEMBL3426947 | — | IC50 | = | 689.0 | nM | 6.16 |
| CHEMBL3658334 | — | IC50 | = | 703.0 | nM | 6.15 |
| CHEMBL3342310 | — | IC50 | = | 759.0 | nM | 6.12 |
| CHEMBL3658246 | — | IC50 | = | 760.0 | nM | 6.12 |
| CHEMBL3658316 | — | IC50 | = | 780.0 | nM | 6.11 |
| CHEMBL3658273 | — | IC50 | = | 852.0 | nM | 6.07 |
| CHEMBL3426952 | — | IC50 | = | 899.0 | nM | 6.05 |
| CHEMBL3342321 | — | IC50 | = | 890.0 | nM | 6.05 |
| CHEMBL3658303 | — | IC50 | = | 981.0 | nM | 6.01 |
| CHEMBL3654442 | — | IC50 | = | 1060.0 | nM | 5.97 |
| CHEMBL3658288 | — | IC50 | = | 1150.0 | nM | 5.94 |
| CHEMBL3658349 | — | IC50 | = | 1190.0 | nM | 5.92 |
| CHEMBL3342290 | — | IC50 | = | 1210.0 | nM | 5.92 |
| CHEMBL3654445 | — | IC50 | = | 1230.0 | nM | 5.91 |
| CHEMBL3658348 | — | IC50 | = | 1230.0 | nM | 5.91 |
| CHEMBL3658336 | — | IC50 | = | 1230.0 | nM | 5.91 |
| CHEMBL3658311 | — | IC50 | = | 1250.0 | nM | 5.90 |
| CHEMBL3342311 | — | IC50 | = | 1480.0 | nM | 5.83 |