Assay Detail
Binding
CHEMBL3705980
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition Assay: Assay method was adopted from a published protocol with some modifications to suit our requirements (Dmitry N Grigoryev et al, Analytical Biochemistry, (1999) 267, 319-330). The conversion of 17-alpha -hydroxy pregnenolone to Dehydroepiandrosterone is accompanied by the release of acetic acid. In the Cyp17 lyase assay, 17-alpha -hydroxy pregnenolone labeled with tritium (3H) at position 21 is used as the substrate. Chloroform extraction removes the radioactive steroids and acetic acid is taken into aqueous layer. The tritiated acetic acid released in the assay thus extracted is quantified to determine the enzyme activity.Initial buffer conditions were, 50 mM Phosphate buffer, pH 7.5 was used as the starting buffer for Cyp17 lyase activity based on the data published in US patent publication No. US2004/0198773 A1. This buffer was found to be suitable for regular Cyp17 lyase assay. Human Cyp 17 gene was cloned and expressed in Adenoviral expression system in A549 cell lines.
50
Total Activities
50
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | A549 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Steroid 17-alpha-hydroxylase/17,20 lyase (CHEMBL3522) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
17α-hydroxylase/C17,20-lyase inhibitors
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 50 | 7.57 | 8.72 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3677969 | — | — | 1 | 8.72 |
| CHEMBL3677940 | — | — | 1 | 8.57 |
| CHEMBL3677971 | — | — | 1 | 8.32 |
| CHEMBL3677960 | — | — | 1 | 8.30 |
| CHEMBL3677970 | — | — | 1 | 8.28 |
| CHEMBL3677947 | — | — | 1 | 8.22 |
| CHEMBL3677927 | — | — | 1 | 8.22 |
| CHEMBL3677951 | — | — | 1 | 8.22 |
| CHEMBL3677972 | — | — | 1 | 8.22 |
| CHEMBL3677936 | — | — | 1 | 8.17 |
| CHEMBL3677955 | — | — | 1 | 8.16 |
| CHEMBL3677937 | — | — | 1 | 8.15 |
| CHEMBL3677943 | — | — | 1 | 8.15 |
| CHEMBL3639767 | — | — | 1 | 8.10 |
| CHEMBL3677924 | — | — | 1 | 8.05 |
| CHEMBL3677968 | — | — | 1 | 8.00 |
| CHEMBL3677934 | — | — | 1 | 7.96 |
| CHEMBL3677962 | — | — | 1 | 7.92 |
| CHEMBL3677953 | — | — | 1 | 7.92 |
| CHEMBL3677928 | — | — | 1 | 7.89 |
| CHEMBL3677957 | — | — | 1 | 7.89 |
| CHEMBL3677926 | — | — | 1 | 7.89 |
| CHEMBL3677942 | — | — | 1 | 7.77 |
| CHEMBL3677945 | — | — | 1 | 7.72 |
| CHEMBL3677965 | — | — | 1 | 7.66 |
| CHEMBL3677930 | — | — | 1 | 7.66 |
| CHEMBL3677925 | — | — | 1 | 7.58 |
| CHEMBL3677935 | — | — | 1 | 7.58 |
| CHEMBL3677959 | — | — | 1 | 7.57 |
| CHEMBL3677946 | — | — | 1 | 7.52 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3677969 | — | IC50 | = | 1.9 | nM | 8.72 |
| CHEMBL3677940 | — | IC50 | = | 2.7 | nM | 8.57 |
| CHEMBL3677971 | — | IC50 | = | 4.75 | nM | 8.32 |
| CHEMBL3677960 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL3677970 | — | IC50 | = | 5.2 | nM | 8.28 |
| CHEMBL3677947 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL3677927 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL3677951 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL3677972 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL3677936 | — | IC50 | = | 6.7 | nM | 8.17 |
| CHEMBL3677955 | — | IC50 | = | 6.9 | nM | 8.16 |
| CHEMBL3677937 | — | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL3677943 | — | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL3639767 | — | IC50 | = | 8.0 | nM | 8.10 |
| CHEMBL3677924 | — | IC50 | = | 9.0 | nM | 8.05 |
| CHEMBL3677968 | — | IC50 | = | 10.0 | nM | 8.00 |
| CHEMBL3677934 | — | IC50 | = | 11.0 | nM | 7.96 |
| CHEMBL3677962 | — | IC50 | = | 12.0 | nM | 7.92 |
| CHEMBL3677953 | — | IC50 | = | 12.0 | nM | 7.92 |
| CHEMBL3677928 | — | IC50 | = | 13.0 | nM | 7.89 |
| CHEMBL3677957 | — | IC50 | = | 13.0 | nM | 7.89 |
| CHEMBL3677926 | — | IC50 | = | 13.0 | nM | 7.89 |
| CHEMBL3677942 | — | IC50 | = | 17.0 | nM | 7.77 |
| CHEMBL3677945 | — | IC50 | = | 19.0 | nM | 7.72 |
| CHEMBL3677930 | — | IC50 | = | 22.0 | nM | 7.66 |
| CHEMBL3677965 | — | IC50 | = | 22.0 | nM | 7.66 |
| CHEMBL3677925 | — | IC50 | = | 26.0 | nM | 7.58 |
| CHEMBL3677935 | — | IC50 | = | 26.0 | nM | 7.58 |
| CHEMBL3677959 | — | IC50 | = | 27.0 | nM | 7.57 |
| CHEMBL3677946 | — | IC50 | = | 30.0 | nM | 7.52 |
| CHEMBL3677966 | — | IC50 | = | 36.0 | nM | 7.44 |
| CHEMBL3677929 | — | IC50 | = | 41.0 | nM | 7.39 |
| CHEMBL3677931 | — | IC50 | = | 42.0 | nM | 7.38 |
| CHEMBL3677956 | — | IC50 | = | 45.0 | nM | 7.35 |
| CHEMBL3677961 | — | IC50 | = | 48.0 | nM | 7.32 |
| CHEMBL3677938 | — | IC50 | = | 58.0 | nM | 7.24 |
| CHEMBL3677964 | — | IC50 | = | 70.0 | nM | 7.16 |
| CHEMBL3677939 | — | IC50 | = | 76.0 | nM | 7.12 |
| CHEMBL3677963 | — | IC50 | = | 84.0 | nM | 7.08 |
| CHEMBL3677933 | — | IC50 | = | 99.0 | nM | 7.00 |
| CHEMBL3677941 | — | IC50 | = | 106.0 | nM | 6.97 |
| CHEMBL3677932 | — | IC50 | = | 121.0 | nM | 6.92 |
| CHEMBL3677950 | — | IC50 | = | 161.0 | nM | 6.79 |
| CHEMBL3677952 | — | IC50 | = | 169.0 | nM | 6.77 |
| CHEMBL3677954 | — | IC50 | = | 184.0 | nM | 6.74 |
| CHEMBL3677958 | — | IC50 | = | 341.0 | nM | 6.47 |
| CHEMBL3677967 | — | IC50 | = | 397.0 | nM | 6.40 |
| CHEMBL3677948 | — | IC50 | = | 461.0 | nM | 6.34 |
| CHEMBL3677949 | — | IC50 | = | 548.0 | nM | 6.26 |
| CHEMBL3677944 | — | IC50 | = | 1213.0 | nM | 5.92 |