Assay Detail
Binding
CHEMBL3706282
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Flashplate Assay: The kinase assay is performed as 384-well flashplate assay (for example for Topcount measurement).0.6 nM TANK binding kinase (TBK1), 800 nM biotinylated MELK-derived peptide (Biotin-Ah-Ah-AKPKGNKDYHLQTCCGSLAYRRR) and 10 uM ATP (spiked with 0.25 uCi of 33P-ATP/well) are incubated at 30 C. for 120 min in a total volume of 50 ul (10 mM MOPS, 10 mM Mg acetate, 0.1 mM EGTA, 1 mM DTT, 0.02% of Brij35, 0.1% of BSA, pH 7.5) with or without test compound. The reaction is stopped with 25 ul of 200 mM EDTA. After 30 min at room temperature, the liquid is removed, and each well is washed three times with 100 ul of 0.9% sodium chloride solution. Non-specific reaction is measured in the presence of 100 nM staurosporine. The radioactivity is measured in a Topcount (PerkinElmer).
93
Total Activities
93
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Target
Serine/threonine-protein kinase TBK1 (CHEMBL5408) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Benzonitrile derivatives as kinase inhibitors
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 93 | 7.22 | 8.70 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3682430 | — | — | 1 | 8.70 |
| CHEMBL3687186 | — | — | 1 | 8.70 |
| CHEMBL3639859 | — | — | 1 | 8.52 |
| CHEMBL3682431 | — | — | 1 | 8.52 |
| CHEMBL3687185 | — | — | 1 | 8.30 |
| CHEMBL3687128 | — | — | 1 | 8.30 |
| CHEMBL3682434 | — | — | 1 | 8.30 |
| CHEMBL3687176 | — | — | 1 | 8.22 |
| CHEMBL3687175 | — | — | 1 | 8.22 |
| CHEMBL3682420 | — | — | 1 | 8.22 |
| CHEMBL3687129 | — | — | 1 | 8.22 |
| CHEMBL3682423 | — | — | 1 | 8.22 |
| CHEMBL3687181 | — | — | 1 | 8.15 |
| CHEMBL3687132 | — | — | 1 | 8.15 |
| CHEMBL3682429 | — | — | 1 | 8.10 |
| CHEMBL3687151 | — | — | 1 | 8.10 |
| CHEMBL3687160 | — | — | 1 | 8.10 |
| CHEMBL3687125 | — | — | 1 | 8.00 |
| CHEMBL3687183 | — | — | 1 | 8.00 |
| CHEMBL3682428 | — | — | 1 | 8.00 |
| CHEMBL3687127 | — | — | 1 | 7.96 |
| CHEMBL3687182 | — | — | 1 | 7.92 |
| CHEMBL3687124 | — | — | 1 | 7.85 |
| CHEMBL3687130 | — | — | 1 | 7.85 |
| CHEMBL3687158 | — | — | 1 | 7.85 |
| CHEMBL3687111 | — | — | 1 | 7.85 |
| CHEMBL3687180 | — | — | 1 | 7.80 |
| CHEMBL3687168 | — | — | 1 | 7.75 |
| CHEMBL3682427 | — | — | 1 | 7.68 |
| CHEMBL3687153 | — | — | 1 | 7.68 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3682430 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3687186 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3639859 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3682431 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3687185 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL3687128 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL3682434 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL3687176 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL3687175 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL3682420 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL3687129 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL3682423 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL3687181 | — | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL3687132 | — | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL3687160 | — | IC50 | = | 8.0 | nM | 8.10 |
| CHEMBL3682429 | — | IC50 | = | 8.0 | nM | 8.10 |
| CHEMBL3687151 | — | IC50 | = | 8.0 | nM | 8.10 |
| CHEMBL3687125 | — | IC50 | = | 10.0 | nM | 8.00 |
| CHEMBL3687183 | — | IC50 | = | 10.0 | nM | 8.00 |
| CHEMBL3682428 | — | IC50 | = | 10.0 | nM | 8.00 |
| CHEMBL3687127 | — | IC50 | = | 11.0 | nM | 7.96 |
| CHEMBL3687182 | — | IC50 | = | 12.0 | nM | 7.92 |
| CHEMBL3687111 | — | IC50 | = | 14.0 | nM | 7.85 |
| CHEMBL3687158 | — | IC50 | = | 14.0 | nM | 7.85 |
| CHEMBL3687124 | — | IC50 | = | 14.0 | nM | 7.85 |
| CHEMBL3687130 | — | IC50 | = | 14.0 | nM | 7.85 |
| CHEMBL3687180 | — | IC50 | = | 16.0 | nM | 7.80 |
| CHEMBL3687168 | — | IC50 | = | 18.0 | nM | 7.75 |
| CHEMBL3682427 | — | IC50 | = | 21.0 | nM | 7.68 |
| CHEMBL3687153 | — | IC50 | = | 21.0 | nM | 7.68 |
| CHEMBL3687138 | — | IC50 | = | 23.0 | nM | 7.64 |
| CHEMBL3687126 | — | IC50 | = | 24.0 | nM | 7.62 |
| CHEMBL3687133 | — | IC50 | = | 24.0 | nM | 7.62 |
| CHEMBL3682432 | — | IC50 | = | 27.0 | nM | 7.57 |
| CHEMBL3687155 | — | IC50 | = | 30.0 | nM | 7.52 |
| CHEMBL3682433 | — | IC50 | = | 30.0 | nM | 7.52 |
| CHEMBL3687178 | — | IC50 | = | 32.0 | nM | 7.50 |
| CHEMBL3687121 | — | IC50 | = | 32.0 | nM | 7.50 |
| CHEMBL3687149 | — | IC50 | = | 34.0 | nM | 7.47 |
| CHEMBL3687136 | — | IC50 | = | 41.0 | nM | 7.39 |
| CHEMBL3687118 | — | IC50 | = | 41.0 | nM | 7.39 |
| CHEMBL3687172 | — | IC50 | = | 42.0 | nM | 7.38 |
| CHEMBL3687137 | — | IC50 | = | 50.0 | nM | 7.30 |
| CHEMBL3687143 | — | IC50 | = | 54.0 | nM | 7.27 |
| CHEMBL3687162 | — | IC50 | = | 55.0 | nM | 7.26 |
| CHEMBL3687113 | — | IC50 | = | 60.0 | nM | 7.22 |
| CHEMBL3687142 | — | IC50 | = | 65.0 | nM | 7.19 |
| CHEMBL3687163 | — | IC50 | = | 70.0 | nM | 7.16 |
| CHEMBL3687184 | — | IC50 | = | 70.0 | nM | 7.16 |
| CHEMBL3687159 | — | IC50 | = | 71.0 | nM | 7.15 |