Assay Detail
Binding
CHEMBL3707601
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibitory Assay: The method for measuring the in-vitro inhibitory activity of a test compound against Aurora B kinase activity was substantially the same as in the case of Aurora A. A purified recombinant human Aurora B protein was purchased from Carna Biosciences, Inc. The composition of a reaction buffer was 20 mM HEPES (pH 7.4), 2 mM DTT, 0.01% Tween-20, magnesium chloride (final concentration: 1 mM) and ATP (final concentration: 40 μM), and incubation time was set to 60 minutes.
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Method for enhancing anti-tumor effect of a microtubule-targeting drug, and a method for treatment of tumor
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 13 | 6.47 | 7.05 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3692218 | — | — | 1 | 7.05 |
| CHEMBL3692206 | TAS-119 | 1.0 | 1 | 6.85 |
| CHEMBL3692210 | — | — | 1 | 6.75 |
| CHEMBL3692215 | — | — | 1 | 6.72 |
| CHEMBL3692209 | — | — | 1 | 6.58 |
| CHEMBL3692207 | — | — | 1 | 6.47 |
| CHEMBL3692214 | — | — | 1 | 6.42 |
| CHEMBL3692217 | — | — | 1 | 6.41 |
| CHEMBL3692216 | — | — | 1 | 6.35 |
| CHEMBL3692211 | — | — | 1 | 6.34 |
| CHEMBL3692213 | — | — | 1 | 6.06 |
| CHEMBL3692212 | — | — | 1 | 6.05 |
| CHEMBL3692208 | — | — | 1 | 6.03 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3692218 | — | IC50 | = | 90.0 | nM | 7.05 |
| CHEMBL3692206 | TAS-119 | IC50 | = | 140.0 | nM | 6.85 |
| CHEMBL3692210 | — | IC50 | = | 180.0 | nM | 6.75 |
| CHEMBL3692215 | — | IC50 | = | 190.0 | nM | 6.72 |
| CHEMBL3692209 | — | IC50 | = | 260.0 | nM | 6.58 |
| CHEMBL3692207 | — | IC50 | = | 340.0 | nM | 6.47 |
| CHEMBL3692214 | — | IC50 | = | 380.0 | nM | 6.42 |
| CHEMBL3692217 | — | IC50 | = | 390.0 | nM | 6.41 |
| CHEMBL3692216 | — | IC50 | = | 450.0 | nM | 6.35 |
| CHEMBL3692211 | — | IC50 | = | 460.0 | nM | 6.34 |
| CHEMBL3692213 | — | IC50 | = | 880.0 | nM | 6.06 |
| CHEMBL3692212 | — | IC50 | = | 900.0 | nM | 6.05 |
| CHEMBL3692208 | — | IC50 | = | 930.0 | nM | 6.03 |