Assay Detail
Binding
CHEMBL3707742
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Kinase Assay: Kinase assays were performed using the assay kit by Reaction Biology Corp (Malvern, Pa.). The compounds were tested at 10 concentrations by 3-fold serial dilutions starting at 30 μM. Staurosporin was used as a control nonspecific kinase inhibitor. In vitro kinase reactions using purified kinases were carried out in the presence of 10 μM ATP and test compounds.
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
5'-AMP-activated protein kinase subunit beta-1 (CHEMBL3847) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Compounds and methods useful for directing stem cell differentiation
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 5 | 6.00 | 6.50 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL478629 | DORSOMORPHIN | — | 1 | 6.50 |
| CHEMBL3668018 | — | — | 1 | 6.28 |
| CHEMBL2385590 | — | — | 1 | 5.95 |
| CHEMBL3668019 | — | — | 1 | 5.71 |
| CHEMBL331465 | — | — | 1 | 5.54 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL478629 | DORSOMORPHIN | IC50 | = | 318.2 | nM | 6.50 |
| CHEMBL3668018 | — | IC50 | = | 527.0 | nM | 6.28 |
| CHEMBL2385590 | — | IC50 | = | 1122.0 | nM | 5.95 |
| CHEMBL3668019 | — | IC50 | = | 1940.0 | nM | 5.71 |
| CHEMBL331465 | — | IC50 | = | 2916.0 | nM | 5.54 |