Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL3707783

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
In vitro Competitive Displacement Binding Assays : Modified SMAC peptides and compounds were tested for their ability to displace the fluorescent tracer from either XIAP, clAP-1 or clAP-2. BIR3 domains of clAP-1 , clAP-2 and XIAP were incubated with test compounds or SMAC based peptides and their respective peptide probes (Peptide Protein Research) in assay buffer (50mM Hepes pH 7.5, 0.025% Tween-20, 0.01 % BSA, and 1 mM DTT). Positive controls consisted of BIR3 proteins and tracer (no inhibition) and negative controls contained tracer only (100% inhibition). The samples were incubated at room temperature for 1 hr (XIAP and clAP-2) or 3hrs (clAP-1 ) prior to being read in the BMG Pherastar in Fluorescence Polarization mode (FP 485nm, 520nm, 520nm).
162
Total Activities
162
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Baculoviral IAP repeat-containing protein 2 (CHEMBL5462)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Bicyclic heterocycle compounds and their uses in therapy
(2015)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 162 6.56 8.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3699960 1 8.05
CHEMBL3700091 1 8.02
CHEMBL3600717 1 8.00
CHEMBL3700047 1 7.96
CHEMBL3700050 1 7.96
CHEMBL3700057 1 7.96
CHEMBL3699967 1 7.92
CHEMBL3700023 1 7.92
CHEMBL3699980 1 7.92
CHEMBL3700040 1 7.89
CHEMBL3700105 1 7.85
CHEMBL3700060 1 7.85
CHEMBL3700016 1 7.85
CHEMBL3700028 1 7.85
CHEMBL3699975 1 7.85
CHEMBL3700042 1 7.85
CHEMBL3699981 1 7.85
CHEMBL3700085 1 7.85
CHEMBL3700080 1 7.85
CHEMBL3699958 1 7.80
CHEMBL3699970 1 7.80
CHEMBL3699959 1 7.80
CHEMBL3699951 1 7.77
CHEMBL3699978 1 7.77
CHEMBL3700025 1 7.77
CHEMBL3700104 1 7.77
CHEMBL3700051 1 7.75
CHEMBL3700029 1 7.72
CHEMBL3699972 1 7.72
CHEMBL3639988 1 7.68

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3699960 IC50 = 9.0 nM 8.05
CHEMBL3700091 IC50 = 9.5 nM 8.02
CHEMBL3600717 IC50 = 10.0 nM 8.00
CHEMBL3700057 IC50 = 11.0 nM 7.96
CHEMBL3700050 IC50 = 11.0 nM 7.96
CHEMBL3700047 IC50 = 11.0 nM 7.96
CHEMBL3699967 IC50 = 12.0 nM 7.92
CHEMBL3699980 IC50 = 12.0 nM 7.92
CHEMBL3700023 IC50 = 12.0 nM 7.92
CHEMBL3700040 IC50 = 13.0 nM 7.89
CHEMBL3700028 IC50 = 14.0 nM 7.85
CHEMBL3700085 IC50 = 14.0 nM 7.85
CHEMBL3699981 IC50 = 14.0 nM 7.85
CHEMBL3700042 IC50 = 14.0 nM 7.85
CHEMBL3699975 IC50 = 14.0 nM 7.85
CHEMBL3700105 IC50 = 14.0 nM 7.85
CHEMBL3700080 IC50 = 14.0 nM 7.85
CHEMBL3700016 IC50 = 14.0 nM 7.85
CHEMBL3700060 IC50 = 14.0 nM 7.85
CHEMBL3699959 IC50 = 16.0 nM 7.80
CHEMBL3699970 IC50 = 16.0 nM 7.80
CHEMBL3699958 IC50 = 16.0 nM 7.80
CHEMBL3699951 IC50 = 17.0 nM 7.77
CHEMBL3699978 IC50 = 17.0 nM 7.77
CHEMBL3700104 IC50 = 17.0 nM 7.77
CHEMBL3700025 IC50 = 17.0 nM 7.77
CHEMBL3700051 IC50 = 18.0 nM 7.75
CHEMBL3700029 IC50 = 19.0 nM 7.72
CHEMBL3699972 IC50 = 19.0 nM 7.72
CHEMBL3700094 IC50 = 21.0 nM 7.68
CHEMBL3700089 IC50 = 21.0 nM 7.68
CHEMBL3639988 IC50 = 21.0 nM 7.68
CHEMBL3700079 IC50 = 22.0 nM 7.66
CHEMBL3699987 IC50 = 23.0 nM 7.64
CHEMBL3700067 IC50 = 23.0 nM 7.64
CHEMBL3699979 IC50 = 23.0 nM 7.64
CHEMBL3699968 IC50 = 24.0 nM 7.62
CHEMBL3700090 IC50 = 24.0 nM 7.62
CHEMBL3700106 IC50 = 24.0 nM 7.62
CHEMBL3700081 IC50 = 24.0 nM 7.62
CHEMBL3699962 IC50 = 25.0 nM 7.60
CHEMBL3700061 IC50 = 29.0 nM 7.54
CHEMBL3700001 IC50 = 29.0 nM 7.54
CHEMBL3700055 IC50 = 31.0 nM 7.51
CHEMBL3700058 IC50 = 32.0 nM 7.50
CHEMBL3699963 IC50 = 33.0 nM 7.48
CHEMBL3699991 IC50 = 35.0 nM 7.46
CHEMBL3699961 IC50 = 35.0 nM 7.46
CHEMBL3700059 IC50 = 40.0 nM 7.40
CHEMBL3700108 IC50 = 41.0 nM 7.39