Assay Detail
Binding
CHEMBL3734107
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of GST/His6-tagged human YAK3 (124 to 526 residues) expressed in baculovirus infected insect Sf9 cells using biotinyl-LGGRDSRAGS*PMARR-OH as substrate after 2 hrs by scintillation proximity assay in presence of [gamma33P]ATP
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Sf9 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Dual specificity tyrosine-phosphorylation-regulated kinase 3 (CHEMBL4575) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Chemical compounds
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 5 | 7.80 | 9.00 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3728543 | — | — | 1 | 9.00 |
| CHEMBL3728359 | — | — | 1 | 9.00 |
| CHEMBL3730504 | — | — | 1 | 8.00 |
| CHEMBL3731253 | — | — | 1 | 7.00 |
| CHEMBL3730389 | — | — | 1 | 6.00 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3728543 | — | IC50 | = | 1.0 | nM | 9.00 |
| CHEMBL3728359 | — | IC50 | = | 1.0 | nM | 9.00 |
| CHEMBL3730504 | — | IC50 | = | 10.0 | nM | 8.00 |
| CHEMBL3731253 | — | IC50 | = | 100.0 | nM | 7.00 |
| CHEMBL3730389 | — | IC50 | = | 1000.0 | nM | 6.00 |