Assay Detail
Binding
CHEMBL3738515
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of Staphylococcus aureus ATCC 35556 SecA1 expressed in Escherichia coli BL21-gammaDE3 assessed as reduction in intrinsic ATPase activity incubated for 3 hrs measured by malachite green colorimetric method
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Staphylococcus aureus |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Target
Protein translocase subunit SecA 1 (CHEMBL3832949) ↗| Type | SINGLE PROTEIN |
| Organism | Staphylococcus aureus (strain NCTC 8325) |
Publication
Evaluation of small molecule SecA inhibitors against methicillin-resistant Staphylococcus aureus.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 3 | 5.05 | 6.00 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL2359093 | ROSE BENGAL SODIUM | 2.0 | 1 | 6.00 |
| CHEMBL3735815 | — | — | 1 | 4.70 |
| CHEMBL3736159 | — | — | 1 | 4.46 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL2359093 | ROSE BENGAL SODIUM | IC50 | = | 1000.0 | nM | 6.00 |
| CHEMBL3735815 | — | IC50 | = | 20000.0 | nM | 4.70 |
| CHEMBL3736159 | — | IC50 | = | 35000.0 | nM | 4.46 |