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Assay Detail

CHEMBL3742870

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to human GST-tagged TNF extracellular domain expressed in Escherichia coli BL21(DE3)pLysS cells by fluorescence spectrophotometric analysis
41
Total Activities
41
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tumor necrosis factor (CHEMBL1825)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and biological evaluation of potential small moleculeinhibitors of tumor necrosis factor
Papaneophytou C, Alexiou P, Papakyriakou A, Ntougkos E, Tsiliouka K, Maranti A, Liepouri F, Strongilos A, Mettou A, Couladouros E, Eliopoulos E, Douni E, Kollias G, Kontopidis G
Medchemcomm (2015) 6 : 1196 -1209
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 41 5.09 6.13

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3740080 1 6.13
CHEMBL3740051 1 6.02
CHEMBL3741883 1 5.79
CHEMBL3741027 1 5.67
CHEMBL3742137 1 5.60
CHEMBL3741275 1 5.50
CHEMBL3741215 1 5.32
CHEMBL3741778 1 5.29
CHEMBL3740564 1 5.28
CHEMBL3740334 1 5.26
CHEMBL3741720 1 5.16
CHEMBL3741926 1 5.11
CHEMBL3741784 1 5.08
CHEMBL3741761 1 5.00
CHEMBL3740763 1 4.99
CHEMBL3741962 1 4.97
CHEMBL3742191 1 4.91
CHEMBL3740702 1 4.89
CHEMBL3742150 1 4.88
CHEMBL3740948 1 4.85
CHEMBL3739810 1 4.82
CHEMBL3741278 1 4.81
CHEMBL3741940 1 4.77
CHEMBL3740629 1 4.76
CHEMBL3740371 1 4.75
CHEMBL3739952 1 4.73
CHEMBL3740299 1 4.73
CHEMBL3741286 1 4.60
CHEMBL3741025 1 4.60
CHEMBL3740423 1 4.45

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3740080 Kd = 740.0 nM 6.13
CHEMBL3740051 Kd = 950.0 nM 6.02
CHEMBL3741883 Kd = 1610.0 nM 5.79
CHEMBL3741027 Kd = 2140.0 nM 5.67
CHEMBL3742137 Kd = 2510.0 nM 5.60
CHEMBL3741275 Kd = 3160.0 nM 5.50
CHEMBL3741215 Kd = 4780.0 nM 5.32
CHEMBL3741778 Kd = 5120.0 nM 5.29
CHEMBL3740564 Kd = 5200.0 nM 5.28
CHEMBL3740334 Kd = 5450.0 nM 5.26
CHEMBL3741720 Kd = 7000.0 nM 5.16
CHEMBL3741926 Kd = 7820.0 nM 5.11
CHEMBL3741784 Kd = 8220.0 nM 5.08
CHEMBL3741761 Kd = 10120.0 nM 5.00
CHEMBL3740763 Kd = 10330.0 nM 4.99
CHEMBL3741962 Kd = 10740.0 nM 4.97
CHEMBL3742191 Kd = 12220.0 nM 4.91
CHEMBL3740702 Kd = 12810.0 nM 4.89
CHEMBL3742150 Kd = 13280.0 nM 4.88
CHEMBL3740948 Kd = 14120.0 nM 4.85
CHEMBL3739810 Kd = 15170.0 nM 4.82
CHEMBL3741278 Kd = 15610.0 nM 4.81
CHEMBL3741940 Kd = 16820.0 nM 4.77
CHEMBL3740629 Kd = 17270.0 nM 4.76
CHEMBL3740371 Kd = 17850.0 nM 4.75
CHEMBL3739952 Kd = 18640.0 nM 4.73
CHEMBL3740299 Kd = 18750.0 nM 4.73
CHEMBL3741286 Kd = 25030.0 nM 4.60
CHEMBL3741025 Kd = 25360.0 nM 4.60
CHEMBL3740423 Kd = 35230.0 nM 4.45
CHEMBL3741437 Kd > 35000.0 nM -
CHEMBL3740816 Kd - -
CHEMBL3740927 Kd - -
CHEMBL3740630 Kd - -
CHEMBL3740824 Kd - -
CHEMBL3741922 Kd > 35000.0 nM -
CHEMBL3741015 Kd - -
CHEMBL3739774 Kd - -
CHEMBL3741299 Kd - -
CHEMBL3739428 Kd - -
CHEMBL3740193 Kd - -