Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL3742872

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human TNF assessed as reduction in TNF-induced cell death of actinomycin D activated mouse L929 cells preincubated for 30 mins followed by cell addition measured after 18 to 24 hrs by crystal violet staining based spectrophotometric analysis
ChEMBL ↗
42
Total Activities
42
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type L929
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tumor necrosis factor (CHEMBL1825) ↗
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and biological evaluation of potential small moleculeinhibitors of tumor necrosis factor
Papaneophytou C, Alexiou P, Papakyriakou A, Ntougkos E, Tsiliouka K, Maranti A, Liepouri F, Strongilos A, Mettou A, Couladouros E, Eliopoulos E, Douni E, Kollias G, Kontopidis G
Medchemcomm (2015) 6 : 1196 -1209
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 42 4.76 5.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL255489 — — 1 5.30
CHEMBL3741761 — — 1 5.00
CHEMBL3741883 — — 1 5.00
CHEMBL3740948 — — 1 5.00
CHEMBL3740080 — — 1 4.82
CHEMBL3741027 — — 1 4.82
CHEMBL3740702 — — 1 4.82
CHEMBL3741940 — — 1 4.70
CHEMBL3741926 — — 1 4.70
CHEMBL3740334 — — 1 4.70
CHEMBL3741275 — — 1 4.70
CHEMBL3740564 — — 1 4.70
CHEMBL3742137 — — 1 4.70
CHEMBL3740051 — — 1 4.60
CHEMBL3739952 — — 1 4.52
CHEMBL3741215 — — 1 4.52
CHEMBL3741278 — — 1 4.40
CHEMBL3741299 — — 1 -
CHEMBL3740193 — — 1 -
CHEMBL3740299 — — 1 -
CHEMBL3739428 — — 1 -
CHEMBL3741962 — — 1 -
CHEMBL3740371 — — 1 -
CHEMBL3741015 — — 1 -
CHEMBL3740763 — — 1 -
CHEMBL3739774 — — 1 -
CHEMBL3742150 — — 1 -
CHEMBL3739810 — — 1 -
CHEMBL3741437 — — 1 -
CHEMBL3740816 — — 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL255489 — IC50 = 5000.0 nM 5.30
CHEMBL3741761 — IC50 = 10000.0 nM 5.00
CHEMBL3741883 — IC50 = 10000.0 nM 5.00
CHEMBL3740948 — IC50 = 10000.0 nM 5.00
CHEMBL3740080 — IC50 = 15000.0 nM 4.82
CHEMBL3741027 — IC50 = 15000.0 nM 4.82
CHEMBL3740702 — IC50 = 15000.0 nM 4.82
CHEMBL3741940 — IC50 = 20000.0 nM 4.70
CHEMBL3741926 — IC50 = 20000.0 nM 4.70
CHEMBL3740334 — IC50 = 20000.0 nM 4.70
CHEMBL3741275 — IC50 = 20000.0 nM 4.70
CHEMBL3740564 — IC50 = 20000.0 nM 4.70
CHEMBL3742137 — IC50 = 20000.0 nM 4.70
CHEMBL3740051 — IC50 = 25000.0 nM 4.60
CHEMBL3741215 — IC50 = 30000.0 nM 4.52
CHEMBL3739952 — IC50 = 30000.0 nM 4.52
CHEMBL3741278 — IC50 = 40000.0 nM 4.40
CHEMBL3741778 — IC50 - — -
CHEMBL3742191 — IC50 - — -
CHEMBL3741025 — IC50 - — -
CHEMBL3740423 — IC50 - — -
CHEMBL3741720 — IC50 > 60000.0 nM -
CHEMBL3741922 — IC50 - — -
CHEMBL3741286 — IC50 - — -
CHEMBL3740629 — IC50 - — -
CHEMBL3740824 — IC50 - — -
CHEMBL3740630 — IC50 - — -
CHEMBL3741784 — IC50 - — -
CHEMBL3740927 — IC50 - — -
CHEMBL3740816 — IC50 - — -
CHEMBL3741437 — IC50 - — -
CHEMBL3739810 — IC50 - — -
CHEMBL3742150 — IC50 - — -
CHEMBL3739774 — IC50 - — -
CHEMBL3740763 — IC50 - — -
CHEMBL3741015 — IC50 - — -
CHEMBL3740371 — IC50 - — -
CHEMBL3741962 — IC50 - — -
CHEMBL3739428 — IC50 - — -
CHEMBL3740299 — IC50 - — -
CHEMBL3740193 — IC50 - — -
CHEMBL3741299 — IC50 - — -