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Assay Detail

CHEMBL3743828

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human TNKS1 (1001 to 1327 residues) expressed in Baculovirus infected Sf9 insect cells using activated DNA as substrate after 1 hr by streptavidin-horseradish peroxidase-based luminescence assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Poly [ADP-ribose] polymerase tankyrase-1 (CHEMBL6164)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of AZ0108, an orally bioavailable phthalazinone PARP inhibitor that blocks centrosome clustering.
Johannes JW, Almeida L, Daly K, Ferguson AD, Grosskurth SE, Guan H, Howard T, Ioannidis S, Kazmirski S, Lamb ML, Larsen NA, Lyne PD, Mikule K, Ogoe C, Peng B, Petteruti P, Read JA, Su N, Sylvester M, Throner S, Wang W, Wang X, Wu J, Ye Q, Yu Y, Zheng X, Scott DA.
Bioorg Med Chem Lett (2015) 25 : 5743 -5747
PubMed 26546219 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 6.42 8.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3740104 1 8.05
CHEMBL521686 OLAPARIB 4.0 1 5.72
CHEMBL3740897 1 5.50

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3740104 IC50 = 9.0 nM 8.05
CHEMBL521686 OLAPARIB IC50 = 1900.0 nM 5.72
CHEMBL3740897 IC50 = 3200.0 nM 5.50