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Assay Detail

CHEMBL3748433

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human PTP1B using pNPP as substrate incubated for 10 mins prior to substrate addition measured after 30 mins by microplate reader analysis
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein phosphatase non-receptor type 1 (CHEMBL335)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

A dimeric urea of the bisabolene sesquiterpene from the Okinawan marine sponge Axinyssa sp. inhibits protein tyrosine phosphatase 1B activity in Huh-7 human hepatoma cells.
Abdjul DB, Kanno S, Yamazaki H, Ukai K, Namikoshi M.
Bioorg Med Chem Lett (2016) 26 : 315 -317
PubMed 26711144 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 5.54 6.14

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL168 OLEANOLIC_ACID 1 6.14
CHEMBL3746974 1 5.72
CHEMBL457458 1 4.77
CHEMBL3747131 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL168 OLEANOLIC_ACID IC50 = 730.0 nM 6.14
CHEMBL3746974 IC50 = 1900.0 nM 5.72
CHEMBL457458 IC50 = 17000.0 nM 4.77
CHEMBL3747131 IC50 > 45000.0 nM -