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Assay Detail

CHEMBL3748699

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiviral activity against HIV-1 expressing JRFL envelope infected in human HeLa67 cells expressing CD4 and CCR5 receptors assessed as inhibition of luciferase activity after 3 days by single cycle pseudotype assay
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Human immunodeficiency virus 1
Cell Type HeLa67
Confidence 1 — Organism
Curated By Autocuration

Target

Human immunodeficiency virus 1 (CHEMBL378)
Type ORGANISM
Organism Human immunodeficiency virus 1

Publication

Inhibitors of HIV-1 attachment: The discovery and structure-activity relationships of tetrahydroisoquinolines as replacements for the piperazine benzamide in the 3-glyoxylyl 6-azaindole pharmacophore.
Swidorski JJ, Liu Z, Yin Z, Wang T, Carini DJ, Rahematpura S, Zheng M, Johnson K, Zhang S, Lin PF, Parker DD, Li W, Meanwell NA, Hamann LG, Regueiro-Ren A.
Bioorg Med Chem Lett (2016) 26 : 160 -167
PubMed 26584882 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 26 9.49 10.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3745996 1 10.70
CHEMBL3745794 1 10.70
CHEMBL3747529 1 10.52
CHEMBL3747691 1 10.22
CHEMBL3746546 1 10.10
CHEMBL3747434 1 10.05
CHEMBL3301620 TEMSAVIR 2.0 1 10.00
CHEMBL3746461 1 10.00
CHEMBL3746003 1 9.89
CHEMBL3746570 1 9.85
CHEMBL3746041 1 9.85
CHEMBL3746085 1 9.85
CHEMBL3745982 1 9.82
CHEMBL3746511 1 9.77
CHEMBL3746649 1 9.43
CHEMBL3746625 1 9.39
CHEMBL3747712 1 9.38
CHEMBL3746318 1 9.36
CHEMBL3745999 1 9.32
CHEMBL3747208 1 9.27
CHEMBL3746727 1 9.21
CHEMBL3746732 1 9.15
CHEMBL3747339 1 8.34
CHEMBL3746785 1 8.04
CHEMBL3746748 1 7.27
CHEMBL3747197 1 7.16

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3745996 EC50 = 0.02 nM 10.70
CHEMBL3745794 EC50 = 0.02 nM 10.70
CHEMBL3747529 EC50 = 0.03 nM 10.52
CHEMBL3747691 EC50 = 0.06 nM 10.22
CHEMBL3746546 EC50 = 0.08 nM 10.10
CHEMBL3747434 EC50 = 0.09 nM 10.05
CHEMBL3301620 TEMSAVIR EC50 = 0.1 nM 10.00
CHEMBL3746461 EC50 = 0.1 nM 10.00
CHEMBL3746003 EC50 = 0.13 nM 9.89
CHEMBL3746570 EC50 = 0.14 nM 9.85
CHEMBL3746041 EC50 = 0.14 nM 9.85
CHEMBL3746085 EC50 = 0.14 nM 9.85
CHEMBL3745982 EC50 = 0.15 nM 9.82
CHEMBL3746511 EC50 = 0.17 nM 9.77
CHEMBL3746649 EC50 = 0.37 nM 9.43
CHEMBL3746625 EC50 = 0.41 nM 9.39
CHEMBL3747712 EC50 = 0.42 nM 9.38
CHEMBL3746318 EC50 = 0.44 nM 9.36
CHEMBL3745999 EC50 = 0.48 nM 9.32
CHEMBL3747208 EC50 = 0.54 nM 9.27
CHEMBL3746727 EC50 = 0.62 nM 9.21
CHEMBL3746732 EC50 = 0.71 nM 9.15
CHEMBL3747339 EC50 = 4.62 nM 8.34
CHEMBL3746785 EC50 = 9.2 nM 8.04
CHEMBL3746748 EC50 = 53.4 nM 7.27
CHEMBL3747197 EC50 = 69.2 nM 7.16