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Assay Detail

CHEMBL3749751

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of monophenolase activity of mushroom tyrosinase using L-tyrosine as substrate preincubated with substrate for 10 mins followed by protein addition measured after 15 mins by spectrophotometric analysis
19
Total Activities
15
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Agaricus bisporus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosinase (CHEMBL3318)
Type SINGLE PROTEIN
Organism Agaricus bisporus

Publication

Highly potent tyrosinase inhibitor, neorauflavane from Campylotropis hirtella and inhibitory mechanism with molecular docking.
Tan X, Song YH, Park C, Lee KW, Kim JY, Kim DW, Kim KD, Lee KW, Curtis-Long MJ, Park KH.
Bioorg Med Chem (2016) 24 : 153 -159
PubMed 26706112 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 5.34 7.52
Inhibition 4 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3747057 2 7.52
CHEMBL3745886 2 5.54
CHEMBL287556 KOJIC ACID 1 4.88
CHEMBL3746218 2 4.74
CHEMBL3746757 2 4.04
CHEMBL3745963 1 -
CHEMBL3745949 1 -
CHEMBL3746021 1 -
CHEMBL3747631 1 -
CHEMBL3747601 1 -
CHEMBL3746555 1 -
CHEMBL3746307 1 -
CHEMBL3745965 1 -
CHEMBL3747773 1 -
CHEMBL3746877 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3747057 IC50 = 30.0 nM 7.52
CHEMBL3745886 IC50 = 2900.0 nM 5.54
CHEMBL287556 KOJIC ACID IC50 = 13200.0 nM 4.88
CHEMBL3746218 IC50 = 18400.0 nM 4.74
CHEMBL3746757 IC50 = 92000.0 nM 4.04
CHEMBL3745949 IC50 > 200000.0 nM -
CHEMBL3746021 IC50 > 200000.0 nM -
CHEMBL3747631 IC50 > 200000.0 nM -
CHEMBL3747601 IC50 > 200000.0 nM -
CHEMBL3746555 IC50 > 200000.0 nM -
CHEMBL3746307 IC50 > 200000.0 nM -
CHEMBL3745965 IC50 > 200000.0 nM -
CHEMBL3747773 IC50 > 200000.0 nM -
CHEMBL3745963 IC50 > 200000.0 nM -
CHEMBL3746218 Inhibition - % -
CHEMBL3747057 Inhibition - % -
CHEMBL3746757 Inhibition - % -
CHEMBL3745886 Inhibition - % -
CHEMBL3746877 IC50 > 200000.0 nM -