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Assay Detail

CHEMBL3750381

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PIM3 kinase (unknown origin) using Biotin-AGAGRSRHSSYPAGT-OH as substrate after 2 hrs by alphascreen assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase pim-3 (CHEMBL5407)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of N-(4-((1R,3S,5S)-3-Amino-5-methylcyclohexyl)pyridin-3-yl)-6-(2,6-difluorophenyl)-5-fluoropicolinamide (PIM447), a Potent and Selective Proviral Insertion Site of Moloney Murine Leukemia (PIM) 1, 2, and 3 Kinase Inhibitor in Clinical Trials for Hematological Malignancies.
Burger MT, Nishiguchi G, Han W, Lan J, Simmons R, Atallah G, Ding Y, Tamez V, Zhang Y, Mathur M, Muller K, Bellamacina C, Lindvall MK, Zang R, Huh K, Feucht P, Zavorotinskaya T, Dai Y, Basham S, Chan J, Ginn E, Aycinena A, Holash J, Castillo J, Langowski JL, Wang Y, Chen MY, Lambert A, Fritsch C, Kauffmann A, Pfister E, Vanasse KG, Garcia PD.
J Med Chem (2015) 58 : 8373 -8386
PubMed 26505898 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 3 8.63 9.64

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2048872 1 9.64
CHEMBL1952329 SGI-1776 1.0 1 7.62
CHEMBL3651966 LGH-447 1.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2048872 Ki = 0.23 nM 9.64
CHEMBL1952329 SGI-1776 Ki = 24.0 nM 7.62
CHEMBL3651966 LGH-447 Ki = 0.009 nM -