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Assay Detail

CHEMBL3751505

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess method
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type RAW264.7
Confidence 1 — Organism
Curated By Autocuration

Target

RAW264.7 (CHEMBL612557)
Type CELL-LINE
Organism Mus musculus

Publication

ent-Abietane-type diterpenoids from the roots of Euphorbia ebracteolata with their inhibitory activities on LPS-induced NO production in RAW 264.7 macrophages.
Liu ZG, Li ZL, Li DH, Li N, Bai J, Zhao F, Meng DL, Hua HM.
Bioorg Med Chem Lett (2016) 26 : 1 -5
PubMed 26615888 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 5.12 6.16

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3747302 1 6.16
CHEMBL404387 JOLKINOLIDE B 1 6.06
CHEMBL3747221 1 5.71
CHEMBL3746044 1 5.35
CHEMBL3746967 1 5.23
CHEMBL3746430 1 5.16
CHEMBL3746737 1 4.85
CHEMBL3745928 1 4.81
CHEMBL6 INDOMETHACIN 4.0 1 4.78
CHEMBL1982058 1 4.60
CHEMBL3746722 1 4.48
CHEMBL389621 HYDROCORTISONE 4.0 1 4.27

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3747302 IC50 = 690.0 nM 6.16
CHEMBL404387 JOLKINOLIDE B IC50 = 880.0 nM 6.06
CHEMBL3747221 IC50 = 1970.0 nM 5.71
CHEMBL3746044 IC50 = 4440.0 nM 5.35
CHEMBL3746967 IC50 = 5940.0 nM 5.23
CHEMBL3746430 IC50 = 6980.0 nM 5.16
CHEMBL3746737 IC50 = 14250.0 nM 4.85
CHEMBL3745928 IC50 = 15450.0 nM 4.81
CHEMBL6 INDOMETHACIN IC50 = 16670.0 nM 4.78
CHEMBL1982058 IC50 = 25000.0 nM 4.60
CHEMBL3746722 IC50 = 33060.0 nM 4.48
CHEMBL389621 HYDROCORTISONE IC50 = 54230.0 nM 4.27