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Assay Detail

CHEMBL3756067

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human wild type RET by radiometric assay in presence of [gamma-33P]-ATP
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Proto-oncogene tyrosine-protein kinase receptor Ret (CHEMBL2041)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

A Pyrazolo[3,4-d]pyrimidin-4-amine Derivative Containing an Isoxazole Moiety Is a Selective and Potent Inhibitor of RET Gatekeeper Mutants.
Yoon H, Kwak Y, Choi S, Cho H, Kim ND, Sim T.
J Med Chem (2016) 59 : 358 -373
PubMed 26652860 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 6.93 8.85

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3753341 1 8.85
CHEMBL3753512 1 8.70
CHEMBL3751904 1 8.30
CHEMBL3752118 1 7.57
CHEMBL3752395 1 7.39
CHEMBL3753958 1 7.39
CHEMBL3752879 1 7.28
CHEMBL3752712 1 7.17
CHEMBL3754346 1 7.13
CHEMBL3753242 1 7.02
CHEMBL3754092 1 6.82
CHEMBL3754442 1 6.72
CHEMBL3751965 1 6.68
CHEMBL3752025 1 6.67
CHEMBL3753903 1 6.62
CHEMBL3753714 1 6.62
CHEMBL3752736 1 6.23
CHEMBL3754247 1 6.04
CHEMBL3752827 1 6.04
CHEMBL3753503 1 5.31
CHEMBL3754195 1 5.08

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3753341 IC50 = 1.4 nM 8.85
CHEMBL3753512 IC50 = 2.0 nM 8.70
CHEMBL3751904 IC50 = 5.0 nM 8.30
CHEMBL3752118 IC50 = 27.0 nM 7.57
CHEMBL3752395 IC50 = 41.0 nM 7.39
CHEMBL3753958 IC50 = 41.0 nM 7.39
CHEMBL3752879 IC50 = 52.0 nM 7.28
CHEMBL3752712 IC50 = 68.0 nM 7.17
CHEMBL3754346 IC50 = 74.0 nM 7.13
CHEMBL3753242 IC50 = 96.0 nM 7.02
CHEMBL3754092 IC50 = 150.0 nM 6.82
CHEMBL3754442 IC50 = 190.0 nM 6.72
CHEMBL3751965 IC50 = 210.0 nM 6.68
CHEMBL3752025 IC50 = 213.0 nM 6.67
CHEMBL3753903 IC50 = 241.0 nM 6.62
CHEMBL3753714 IC50 = 241.0 nM 6.62
CHEMBL3752736 IC50 = 583.0 nM 6.23
CHEMBL3754247 IC50 = 910.0 nM 6.04
CHEMBL3752827 IC50 = 910.0 nM 6.04
CHEMBL3753503 IC50 = 4900.0 nM 5.31
CHEMBL3754195 IC50 = 8330.0 nM 5.08