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Assay Detail

CHEMBL3757929

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at human S1P5 receptor expressed in EDG8-bla U2OS cells incubated for 18 hrs prior to GenBlazer substrate addition by beta-arrestin recruitment assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type U2OS
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sphingosine 1-phosphate receptor 5 (CHEMBL2274)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Late-stage optimization of a tercyclic class of S1P3-sparing, S1P1 receptor agonists.
Horan JC, Kuzmich D, Liu P, DiSalvo D, Lord J, Mao C, Hopkins TD, Yu H, Harcken C, Betageri R, Hill-Drzewi M, Patenaude L, Patel M, Fletcher K, Terenzzio D, Linehan B, Xia H, Patel M, Studwell D, Miller C, Hickey E, Levin JI, Smith D, Kemper RA, Modis LK, Bannen LC, Chan DS, Mac MB, Ng S, Wang Y, Xu W, Lemieux RM.
Bioorg Med Chem Lett (2016) 26 : 466 -471
PubMed 26687487 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 4 6.51 6.67

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3752660 1 6.67
CHEMBL3753300 1 6.47
CHEMBL3753609 1 6.46
CHEMBL3753479 1 6.44

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3752660 EC50 = 215.0 nM 6.67
CHEMBL3753300 EC50 = 340.0 nM 6.47
CHEMBL3753609 EC50 = 350.0 nM 6.46
CHEMBL3753479 EC50 = 360.0 nM 6.44