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Assay Detail

CHEMBL3762006

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human OAT3 expressed in HEK cells assessed as reduction of [3H]-estrone sulfate uptake by radioactivity counting analysis
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Organic anion transporter 3 (CHEMBL1641348)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of N-(4-Fluoro-3-methoxybenzyl)-6-(2-(((2S,5R)-5-(hydroxymethyl)-1,4-dioxan-2-yl)methyl)-2H-tetrazol-5-yl)-2-methylpyrimidine-4-carboxamide. A Highly Selective and Orally Bioavailable Matrix Metalloproteinase-13 Inhibitor for the Potential Treatment of Osteoarthritis.
Ruminski PG, Massa M, Strohbach J, Hanau CE, Schmidt M, Scholten JA, Fletcher TR, Hamper BC, Carroll JN, Shieh HS, Caspers N, Collins B, Grapperhaus M, Palmquist KE, Collins J, Baldus JE, Hitchcock J, Kleine HP, Rogers MD, McDonald J, Munie GE, Messing DM, Portolan S, Whiteley LO, Sunyer T, Schnute ME.
J Med Chem (2016) 59 : 313 -327
PubMed 26653735 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 4.73 5.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL603656 1 5.22
CHEMBL496942 1 4.75
CHEMBL270050 1 4.23

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL603656 IC50 = 6000.0 nM 5.22
CHEMBL496942 IC50 = 18000.0 nM 4.75
CHEMBL270050 IC50 = 59000.0 nM 4.23