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Assay Detail

CHEMBL3776497

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of porcine kidney TNAP using para-nitrophenylphosphate as substrate preincubated for 10 mins at pH 7.8 followed by substrate addition
8
Total Activities
4
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Sus scrofa
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Synthesis of benzofuran derivatives as selective inhibitors of tissue-nonspecific alkaline phosphatase: effects on cell toxicity and osteoblast-induced mineralization.
Marquès S, Buchet R, Popowycz F, Lemaire M, Mebarek S.
Bioorg Med Chem Lett (2016) 26 : 1457 -1459
PubMed 26860736 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 4 4.42 4.50
IC50 4 4.47 4.55

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3774470 2 4.55
CHEMBL1454 LEVAMISOLE 4.0 2 4.39
CHEMBL3774946 2 -
CHEMBL3774723 2 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3774470 IC50 = 28000.0 nM 4.55
CHEMBL3774470 Ki = 32000.0 nM 4.50
CHEMBL1454 LEVAMISOLE IC50 = 41000.0 nM 4.39
CHEMBL1454 LEVAMISOLE Ki = 47000.0 nM 4.33
CHEMBL3774946 Ki - -
CHEMBL3774723 Ki - -
CHEMBL3774723 IC50 > 400000.0 nM -
CHEMBL3774946 IC50 > 400000.0 nM -