Assay Detail
Binding
CHEMBL3776533
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Competitive inhibition of N-terminal His6-tagged KDM4C (1 to 352 residues) (unknown origin) expressed in Escherichia coli Rosetta 2(DE3)pLysS using ARK(Me3)STGGK peptide/alpha-ketoglutarate as substrate/cofactor by FDH coupling-based Lineweaver-Burk plot analysis
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Docking and Linking of Fragments To Discover Jumonji Histone Demethylase Inhibitors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 3 | 7.40 | 8.66 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL316034 | — | — | 1 | 8.66 |
| CHEMBL3775262 | — | — | 1 | 7.37 |
| CHEMBL3775380 | — | — | 1 | 6.17 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL316034 | — | Ki | = | 2.2 | nM | 8.66 |
| CHEMBL3775262 | — | Ki | = | 43.2 | nM | 7.37 |
| CHEMBL3775380 | — | Ki | = | 682.9 | nM | 6.17 |