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Assay Detail

CHEMBL3777369

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of CE2 in human liver microsomes using fluorescein diacetate as substrate preincubated for 10 mins followed by substrate addition measured after 30 mins by LC-UV analysis
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Tissue Liver
Subcellular Microsomes
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cocaine esterase (CHEMBL3180)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis, and structure-activity relationship study of glycyrrhetinic acid derivatives as potent and selective inhibitors against human carboxylesterase 2.
Zou LW, Li YG, Wang P, Zhou K, Hou J, Jin Q, Hao DC, Ge GB, Yang L.
Eur J Med Chem (2016) 112 : 280 -288
PubMed 26900660 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 5.31 7.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3774603 1 7.70
CHEMBL2151605 1 6.18
CHEMBL3775320 1 5.47
CHEMBL3775118 1 5.40
CHEMBL3775391 1 5.40
CHEMBL1551302 BAVACHININ A 1 5.37
CHEMBL1271483 1 5.25
CHEMBL1271536 1 5.24
CHEMBL1231178 1 5.21
CHEMBL487933 1 5.16
CHEMBL1271200 1 5.13
CHEMBL3775916 1 4.85
CHEMBL3775913 1 4.68
CHEMBL207413 ACETOXOLONE -1.0 1 4.39
CHEMBL230006 ENOXOLONE 2.0 1 4.16
CHEMBL493512 1 -
CHEMBL3775481 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3774603 IC50 = 20.0 nM 7.70
CHEMBL2151605 IC50 = 660.0 nM 6.18
CHEMBL3775320 IC50 = 3420.0 nM 5.47
CHEMBL3775118 IC50 = 3990.0 nM 5.40
CHEMBL3775391 IC50 = 3950.0 nM 5.40
CHEMBL1551302 BAVACHININ A IC50 = 4310.0 nM 5.37
CHEMBL1271483 IC50 = 5610.0 nM 5.25
CHEMBL1271536 IC50 = 5700.0 nM 5.24
CHEMBL1231178 IC50 = 6110.0 nM 5.21
CHEMBL487933 IC50 = 6950.0 nM 5.16
CHEMBL1271200 IC50 = 7360.0 nM 5.13
CHEMBL3775916 IC50 = 14210.0 nM 4.85
CHEMBL3775913 IC50 = 20750.0 nM 4.68
CHEMBL207413 ACETOXOLONE IC50 = 40510.0 nM 4.39
CHEMBL230006 ENOXOLONE IC50 = 69260.0 nM 4.16
CHEMBL493512 IC50 > 100000.0 nM -
CHEMBL3775481 IC50 > 100000.0 nM -