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Assay Detail

CHEMBL3779254

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of full length human HALO-tagged KDM4C expressed in human U2OS cells assessed as level of H3K9Me3 demethylation incubated overnight by Hoechst 33342/HaloTag/ Alexa Fluor 647 staining-based image analysis
24
Total Activities
23
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type U2OS
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Lysine-specific demethylase 4C (CHEMBL6175)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Cell Penetrant Inhibitors of the KDM4 and KDM5 Families of Histone Lysine Demethylases. 1. 3-Amino-4-pyridine Carboxylate Derivatives.
Westaway SM, Preston AG, Barker MD, Brown F, Brown JA, Campbell M, Chung CW, Diallo H, Douault C, Drewes G, Eagle R, Gordon L, Haslam C, Hayhow TG, Humphreys PG, Joberty G, Katso R, Kruidenier L, Leveridge M, Liddle J, Mosley J, Muelbaier M, Randle R, Rioja I, Rueger A, Seal GA, Sheppard RJ, Singh O, Taylor J, Thomas P, Thomson D, Wilson DM, Lee K, Prinjha RK.
J Med Chem (2016) 59 : 1357 -1369
PubMed 26771107 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 4.79 5.30
pIC50 2 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3774834 1 5.30
CHEMBL3774408 1 5.20
CHEMBL3775889 1 5.20
CHEMBL3775175 1 5.20
CHEMBL3774859 1 5.20
CHEMBL3774552 1 5.20
CHEMBL3775834 1 5.10
CHEMBL3775237 1 5.10
CHEMBL3775522 1 4.80
CHEMBL3774545 1 4.70
CHEMBL3775957 1 4.50
CHEMBL3775276 1 4.50
CHEMBL3775439 1 4.50
CHEMBL3775612 2 4.40
CHEMBL3775498 1 4.40
CHEMBL3774487 1 4.30
CHEMBL3775110 1 4.20
CHEMBL3775716 1 -
CHEMBL3775151 1 -
CHEMBL3775056 1 -
CHEMBL3774863 1 -
CHEMBL3774615 1 -
CHEMBL3774435 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3774834 IC50 = 5011.87 nM 5.30
CHEMBL3774408 IC50 = 6309.57 nM 5.20
CHEMBL3775175 IC50 = 6309.57 nM 5.20
CHEMBL3774859 IC50 = 6309.57 nM 5.20
CHEMBL3774552 IC50 = 6309.57 nM 5.20
CHEMBL3775889 IC50 = 6309.57 nM 5.20
CHEMBL3775237 IC50 = 7943.28 nM 5.10
CHEMBL3775834 IC50 = 7943.28 nM 5.10
CHEMBL3775522 IC50 = 15848.93 nM 4.80
CHEMBL3774545 IC50 = 19952.62 nM 4.70
CHEMBL3775439 IC50 = 31622.78 nM 4.50
CHEMBL3775957 IC50 = 31622.78 nM 4.50
CHEMBL3775276 IC50 = 31622.78 nM 4.50
CHEMBL3775612 IC50 = 40000.0 nM 4.40
CHEMBL3775612 IC50 = 39810.72 nM 4.40
CHEMBL3775498 IC50 = 39810.72 nM 4.40
CHEMBL3774487 IC50 = 50118.72 nM 4.30
CHEMBL3775110 IC50 = 63095.73 nM 4.20
CHEMBL3775716 IC50 > 100000.0 nM -
CHEMBL3775056 pIC50 - -
CHEMBL3774863 pIC50 - -
CHEMBL3774615 IC50 > 100000.0 nM -
CHEMBL3774435 IC50 > 100000.0 nM -
CHEMBL3775151 IC50 > 100000.0 nM -